A novel noninvasive microwave biosensor for glucose measurement was designed, developed and tested to show its potential for real-time and continuous glucose monitoring. The reflected S-parameter S11 was investigated with different glucose concentrations within the range of 0.5 ~ 7 g/dL. The magnitude and resonance frequency information were utilized for prediction and then combined by data fusion to enhance the accuracy. The prediction results by magnitude and resonance frequency of S11 versus reference glucose concentration show root-mean-squared errors (RMSE) of 0.2887 g/dL and 0.1681 g/dL respectively. The prediction by data fusion shows RMSE value of 0.1364 g/dL indicating significant accuracy improvement of 52.75% and 18.86% compared to single parameter based magnitude and frequency predictions.