The Asymmetric Crosstalk Harnessed Signaling (ACHS) scheme [1]–[4] provides a significant improvement to the original Crosstalk Harnessed Signaling (CHS) technique [5]. This paper presents a study of design optimizations applied on 3D multilayer interconnect arrangements [2] [3] [6], in which alterations on the encoding matrices or the distribution of wires in the channel translate into a modification of the bit encoding regions and their associated performances. While ACHS has been presented as a vastly superior signaling option than the originally proposed CHS due to the elimination of the highly sensitive common encoding eigenmode [1], further encoding optimizations on the critical (worst performing) data bits and the strobe inside the ACHS implementation can further improve the bus performance. This work presents a full set of performance comparisons involving data and strobe bits, on a ACHS-encoded bus with channels adjacently routed in 3- and 4-layer 3D interconnect arrangements.