Nowadays, the impact of e-Learning developments on improving educational activities is becoming more evident. The Portfolio approach has emerged as important alternative to increase the learning process. However, most of tools to support portfolio assessment are far from to come up to the expectations. In this work we propose a fuzzy ontology-based framework to support portfolio assessment. Our approach focuses on portfolio semantic representation and conceptual matching to generate a portfolio evaluation report, which helps teachers in portfolio assessment tasks. The initial experiments results indicate that the approach is useful and warrants further research.