The current study sought to detect and quantify different folic acid concentrations in Phosphate Buffer Saline (PBS) by employing Electrical Impedance Spectroscopy (EIS).This technique provides a simple, rapid, precise and cost-effective platform for folic acid monitoring. An on-wafer platform has been developed to perform the necessary electrical measurements. Variation of electrical parameters such as impedance, capacitance and conductance for six different concentrations ranging from 4 mg/dl to 6 mg/dl are analyzed. The capacitance and conductance are observed to increase whereas impedance has been found to decrease with increasing folic acid content in saline. FT-MIR spectrum shows distinct confirmatory peaks relevant to the folic acid constituents in the IR range 4000 cm^-1 to 400 cm^-1.