In the polling-based WiMAX network, the number of served mobile station (MSs) and number of polls (NOP) are the parameters that significantly affects the network performance. Both factors had an impact even on basic the service and arrival rate plays a role in the performance. In this paper, we present the performance analysis of throughput based on the Mobile Multi-hop Relay (MMR) WiMAX. We modelled and analyzed the throughput of the uplink sub-frame using M/M/1 queueing. Numerical result has shown that the throughput increases with number of polls while it decreasing trend when the number of mobile station increased. On the other hand, the throughput starts to saturate as the number of MS increases above 20. Moreover, the throughput of any number of polls in this condition does not vary continuously when it became constant although the numbers of mobile stations increasing.