The perception of the spatial effect of 3D video is influenced by various factors. One of the most interesting and important factors is the content of the observed video sequence. This factor is relatively little investigated. The content of the scene can be described by particular parameters. The parameters have to describe spatial, temporal, depth and brightness distribution in sequences. We have created a database of the stereo video sequences complete with their quantified parameters, which express their contents. The sequences should serve for execution of subjective tests dealing with the impact of video sequence parameters on the observers' perception. We have executed a subjective test with this database.