Ultrasonic devices are today widely used in hospitals for diagnosis and treatment purposes. Quality controls of ultrasonic devices used in the field of health must be performed on phantoms that simulate human tissue with similar acoustic properties to human tissue in terms of both safety and proximity to reality. The phantoms are very important materials and widely used in the calibration of ultrasound devices, performance tests, improvement of signal and noise ratios of existing systems, practical application of the users before the device use and interpretation of the images taken during the application and it is used in the characterization of medical ultrasonic systems since the 1960s. In addition, these phantoms are the reference material for calibration operations. In this study, acoustic characterization measurements were made for three different tissue-mimicking materials and the results obtained were compared with each other.