This paper reports a simple method to capture and rotate a bio-object at a controllable speed in direction of vertical plane. This method intends to implement its function without contact in an open space for biological application such as dynamically monitoring, orientation setting, and mechanical stimulation induced by fluidic shear force. To verify this principle, calculation process of necessary flow rate for generating enough operating force on the cell is conducted with CFD. An open-space microchip with several orifices (diameter is 100 μm) is designed and fabricated. Then the manipulating demonstrations are conducted. On the calculated conditions, the rotation of target cell is successfully obtained. The relation between the object's velocity, flow rate, and operating force is clarified.