3D pH measurement for cell culture using CT scan | IEEE Conference Publication | IEEE Xplore

Abstract:

In cell culture, the pH of culture liquid is one of the most important conditions. However, culture liquid may have pH-gradient due to the activity of cells and change of...Show More

Abstract:

In cell culture, the pH of culture liquid is one of the most important conditions. However, culture liquid may have pH-gradient due to the activity of cells and change of the environment. In order to confirm the existence of pH-gradient, we propose a method for 3D pH measurement using the color change of phenol red. Using the principle of CT scan, we can measure pH-gradient without contacting culture liquid, and thus, decrease the possibility of contamination. In this paper, we consider the influence of light refraction by a cylindrical vessel, and show that we can measure local pH of culture liquid by light absorbance.
Date of Conference: 03-06 December 2017
Date Added to IEEE Xplore: 01 March 2018
ISBN Information:
Electronic ISSN: 2474-3771
Conference Location: Nagoya, Japan

References

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