Image Processing and Analysis for Single-Molecule Localization Microscopy: Computation for nanoscale imaging | IEEE Journals & Magazine | IEEE Xplore

Image Processing and Analysis for Single-Molecule Localization Microscopy: Computation for nanoscale imaging


Abstract:

Fluorescence microscopy is currently the most important tool for visualizing biological structures at the sub?cellular scale. The combination of fluorescence, which enabl...Show More

Abstract:

Fluorescence microscopy is currently the most important tool for visualizing biological structures at the sub?cellular scale. The combination of fluorescence, which enables a high imaging contrast, and the possibility to apply molecular labeling, which allows for a high imaging specificity, makes it a powerful imaging modality. The use of fluorescence microscopy has risen tremendously, in particular since the introduction of the green fluorescent protein (GFP) in the mid-1990s and the possibility to genetically engineer cells to express these proteins. Figure 1 shows the basic layout of a fluorescence microscope. Excitation light of a certain wavelength is reflected via a dichroic beamsplitter and projected onto the specimen via the objective lens of the microscope. The light is absorbed by the fluorescent labels and re-emitted, slightly Stokes-shifted by ?100 nm, at a larger wavelength, typically a few nanoseconds later. The emission light is captured by the objective lens and directed toward the camera via the dichroic beamsplitter.
Published in: IEEE Signal Processing Magazine ( Volume: 32, Issue: 1, January 2015)
Page(s): 49 - 57
Date of Publication: 04 December 2014

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