Based on a novel micro-fabricated electroporation (EP) chip, a portable EP system was designed and manufactured. The whole system consisted of a programmable and adjustable mini impulsator and micro EP chips. Large statistical data of transfection efficiency were determined under various pulse amplitudes, durations and intervals in this system for HEK-293 cells. High transfeciton efficiency (80%) was achieved under low voltage (~20V) in this system. A man-machine interface was developed by keyboard and LCD screen in the mini impulsator using single chip micyoco. The impulsator can be adjusted to accommodate its pulse electrical parameters to a variety of conditions. The system has been fabricated and is in probation. With further EP experiments on other kinds of cells, more guidance will be given for researchers to shorten EP time and enhance efficiency.