A microfluidic device for automatic embryo trapping and coculture with stromal cells in vitro | IEEE Conference Publication | IEEE Xplore

A microfluidic device for automatic embryo trapping and coculture with stromal cells in vitro


Abstract:

Nowadays, infertility is one of the major problems for many of married couples. Thus, there are a wide range of researches on infertility where the in vitro fertilization...Show More

Abstract:

Nowadays, infertility is one of the major problems for many of married couples. Thus, there are a wide range of researches on infertility where the in vitro fertilization is the best known. However, the conventional approach to fertilize embryo requires more on manual operation times that may causes some damage of embryos. To overcome the drawback of conventional method, we present a microfluidic chip to trap cells and mimic a uterus in vitro by co-culturing stromal cells and mouse embryos. By dynamic resistance design, mouse embryos can be trapped individually. In this way, we can grow them by co-culturing with stromal cells in an open system with constant medium supply, known as dynamic perfusion. The novel method can reduce the manual operation and the possibility of damage to embryos. Also, the chip can track and manage individual embryos easily.
Date of Conference: 13-16 April 2014
Date Added to IEEE Xplore: 25 September 2014
Electronic ISBN:978-1-4799-4726-3
Conference Location: Waikiki Beach, HI, USA

References

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