This paper reports the design, fabrication and characterization of an impedance based biosensor for simultaneous detection of three Salmonella serogroups (type B, D, and E), with low concentration in ready to eat Turkey matrix. The measurements demonstrate that the device can selectively detect Salmonella, e.g., type B at a concentration as low as 8 cell/ml with a detection time of 1 hour. The addition of focusing region has improved the signal strength by a factor ranged between 4. The antibody immobilization on top of the detection electrodes was studied as a function of time between 0.5 - 3 hours. The results demonstrate that an optimized coating can be achieved within 1.5 hours at 1X antibody coating. The design has shown a significant improvement in capability to detect Salmonella compared to traditional culture methods, PCR and ELISA based detection.