To further investigate the communication in astrocytic networks, in vitro astrocytes were patterned in simple networks by using parylene-C/SiO2 platform. We designed a 2×2 small network on parylene-C/SiO2 platform and demonstrated, for the first time, that the intracellular calcium response of a single human hNT astrocyte stimulated by UV laser pulses can be transmitted to neighboring astrocytes in this 2×2 small network. The calcium responses of the astrocytes network were recorded and analyzed.