This paper deals with crosstalk distortion (or coupling) between the multi-channel RF components in the wideband multi-input-multi-output (MIMO) transceiver systems. We study the feasible crosstalk parameter estimation, compensation techniques, and calibration procedures in the coupling scenario of the joint transmitter (TX) and receiver (RX). For the MIMO wideband crosstalk transceiver, the proposed calibration rule includes the wideband crosstalk estimation, followed by the compensation procedures of “RX calibration” and “TX calibration”. Next, the printed circuit board (PCB) with crosstalk effect is adopted to verify the performance of the proposed crosstalk distortion compensation. Then, it can be realized in the transmission of single-carrier and multi-carrier orthogonal frequency division multiplexing (OFDM) signals with decoupling processing. Furthermore, to achieve the above purpose, this paper uses the commercial AD9371 wideband RF module integrated with the wideband actual crosstalk PCB module. Finally, the measurement results show that the wideband crosstalk estimation and compensation techniques can suppress crosstalk about 30dB when applied to single-carrier signals, and the error vector measurement (EVM) performance can be improved about 20dB when applied to OFDM signals. Therefore, the proposed design methodology is useful and can provide high-quality performance for wideband MIMO communication.