In many cryptographic systems, a random number generator is an essential component. Because random numbers are unpredictable, they are required in these systems. This generated number is either used as the cryptographic algorithm's key or as a random nonce. Various true random number generator has already existing. Among them the most hardware resource efficient solutions are Physical unclonable Function (PUF) based solution. In this paper use of one such resource efficient solution has been proposed which is implemented on a zynq ultralscale+ MPSoC chip and on zynq 7000 series soc chip(Zedboard) with minimum resource utilization. The 64 bit PUF design uses 280 look-up-tables(LUTs) on zynq MPSsoC board and 302 LUTs on Zedboard and340 registers for both. Also the collected responses show good stability at different times and with temperature of around 20°C −30°C.