Since the number of trans-femoral amputees has been increased by industrial or traffic accidents in modern society, a prosthetic limb as typified by trans-femoral prosthesis has been required. On the other hand, the sprinting motion of unilateral trans-femoral amputee sprinters has been investigated to determine a direction for training with high level at maximal velocity. However, structural optimization of the prosthetic foot for the jumping movements continued from sprinting motion based on experimental evidence consistent with biomechanics has not been investigated yet. In this paper, we aim to optimize the structure of the prosthetic foot for the jumping movements of a trans-femoral amputee considering ground reaction forces based on biomechanical evidence as each contact condition to the floor. As a result of applying proposed multiple objective optimization technique using response surface method and desirability function to the prosthetic foot by finite element analysis, we obtained optimum design variables. Finally, we improved the structure of the prosthetic foot by obtaining sufficient larger deflections and optimum design variables based on the evaluation of the simulation results and showed the effectiveness of the structural optimization concerning the prosthetic foot.