Human-Computer Interaction (HCI) and Machine Learning (ML) technologies have potential for the behavioral screening of autistic children but how to design a tool and analyse behavior reliably is challenging. Based on psychophysiological computation, this paper proposes an interactive behavior perception analytical model for autism screening. We presented the multi-scenario reactive behavior paradigms that designed based on the atypical characteristics of autistic children. We recorded the eye movement data and facial data of 91 participants, and performed multi-modal feature extraction, used machine learning to train classification model. We conducted comparative experiments, and the experimental results verified the advantages of multi-scenario paradigms and multi-modal feature groups, which indicates that our analysis methods and screening models are effective and reliable and have real research significance.