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A Two-Dimensional Lock-In Algorithm for Signal Analysis in Patterned Images | IEEE Journals & Magazine | IEEE Xplore

A Two-Dimensional Lock-In Algorithm for Signal Analysis in Patterned Images


Abstract:

Goal: We extend a classic signal processing algorithm, the lock-in amplifier, to two dimensions to extract the signal in patterned images. Methods: The algorithm was eval...Show More

Abstract:

Goal: We extend a classic signal processing algorithm, the lock-in amplifier, to two dimensions to extract the signal in patterned images. Methods: The algorithm was evaluated using simulated image data and experimental microscopy images to determine the fluorescence signal of fluorescently labeled proteins adsorbed on surfaces patterned with chemical vapor deposition. Results: The algorithm was capable of retrieving the signal with a signal-to-noise ratio as low as -20 dB. Optimal parameters were determined for the pattern design. Conclusion: The lock-in algorithm is a powerful technique for 1/f noise removal. Significance: The methodology holds promise not only for the measurement of adsorption events on patterned surfaces but in all situations where a signal has to be extracted from a noisy background in two or more dimensions.
Published in: IEEE Transactions on Biomedical Engineering ( Volume: 63, Issue: 11, November 2016)
Page(s): 2436 - 2440
Date of Publication: 08 February 2016

ISSN Information:

PubMed ID: 26886961

Funding Agency:


References

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