Processing math: 100%
Ultrawide Range of Concentration Measurement Assisted by Fluorescence Intensity Distribution Imaging | IEEE Journals & Magazine | IEEE Xplore

Ultrawide Range of Concentration Measurement Assisted by Fluorescence Intensity Distribution Imaging


Abstract:

When performing fluorescence measurement on a fluorescent analyte over an ultrawide concentration range, the fluorescence intensity distribution is affected by the spatia...Show More

Abstract:

When performing fluorescence measurement on a fluorescent analyte over an ultrawide concentration range, the fluorescence intensity distribution is affected by the spatial attenuation effect of excitation light, causing the linear relationship between the received fluorescence intensity and the fluorophore concentration to no longer hold. In this work, based on the imaging analysis of fluorescence intensity spatial distribution, the spatial invariant ( \kappa ) is defined in the form of relative fluorescence intensity, and the other spatial invariant ( \mu ) is given in the form of spatial differential accordingly. The use of the spatial invariants instead of the absolute fluorescence intensity commonly used to reflect the fluorophore concentration can get rid of the dependence of geometrical parameters and can also avoid systematic errors caused by unstable measurement conditions. Taking these spatial invariants as parameters has great advantages in ultrawide range of fluorescence measurement (0.02~125.00 mg/L) and produces more accurate concentration results ( R^{2}=0.9975 and \mathrm {RRMSE}=0.0510 ).
Article Sequence Number: 9512706
Date of Publication: 04 September 2023

ISSN Information:

Funding Agency:


References

References is not available for this document.