Radio frequency (RF) channel reciprocity calibration is one of the most important technologies in massive multiple-input multiple-output (MIMO) system. With the increasing of the antenna size, the calibration procedure becomes much more challenging. In this paper, wired- and wireless-calibration schemes have been proposed and investigated through field-trial test based on a real-time massive MIMO prototype with 64 independent RF channels. The prototype is capable of supporting 12 200-MHz user equipments (UEs) simultaneously. The field trial test results validate both schemes can significantly improve the overall system throughput. For single-user (SU) MIMO scenario, the throughput can be enhanced from 0.71 Gbps to 1.46 Gbps and 1.54 Gbps with wireless- and wired-calibration schemes, respectively. For multi-user (MU) MIMO scenario, the corresponding enhancement can be from 0.26 Gbps to 9.18 Gbps and 11.29 Gbps, respectively. In terms of calibration accuracy, the wired-calibration scheme has better performance, but the wireless one has lower system complexity and cost. To the best of our knowledge, this is the first field trial investigation of wireless-calibration scheme and comparison with wired calibration in a real-time high-throughput massive MIMO prototype.