The existing localization methods for the satellite communication interference source based on satellites are too complex and costly to be used in practical applications. A new localization method based on two satellites for the interference source is proposed in this paper. By combining two localization parameters named time difference of arrival (TDOA) between two satellites and the direction of arrival(DOA) from the main satellite, the three-dimensional locations of the interference source can be achieved. The three-dimensional mathematical localization model for the interference source is established with the vector solution method. Moreover, in Monte Carlo analysis, the localization error model is established in consideration of many error factors. Lastly, the proposed localization technology is used in the suppositional scene which is composed of a main satellite and an auxiliary satellite, and the numerical simulation is carried out. The simulation results show that the proposed localization technology can realize accurate estimation of the ground track and the three-dimensional position for the interference source even if it is aerial moving with a turning maneuver.