Automatic glandular and tubule region segmentation in histological grading of breast cancer

Kien Nguyen; Michael Barnes; Chukka Srinivas; Christophe Chefd'hotel

doi:10.1117/12.2082322

19 March 2015 Automatic glandular and tubule region segmentation in histological grading of breast cancer

Kien Nguyen, Michael Barnes, Chukka Srinivas, Christophe Chefd'hotel

Proceedings Volume 9420, Medical Imaging 2015: Digital Pathology; 94200G (2015) https://doi.org/10.1117/12.2082322
Event: SPIE Medical Imaging, 2015, Orlando, Florida, United States

Abstract

In the popular Nottingham histologic score system for breast cancer grading, the pathologist analyzes the H and E tissue slides and assigns a score, in the range of 1-3, for tubule formation, nuclear pleomorphism and mitotic activity in the tumor regions. The scores from these three factors are added to give a final score, ranging from 3-9 to grade the cancer. Tubule score (TS), which reflects tubular formation, is a value in 1-3 given by manually estimating the percentage of glandular regions in the tumor that form tubules. In this paper, given an H and E tissue image representing a tumor region, we propose an automated algorithm to detect glandular regions and detect the presence of tubules in these regions. The algorithm first detects all nuclei and lumen candidates in the input image, followed by identifying tumor nuclei from the detected nuclei and identifying true lumina from the lumen candidates using a random forest classifier. Finally, it forms the glandular regions by grouping the closely located tumor nuclei and lumina using a graph-cut-based method. The glandular regions containing true lumina are considered as the ones that form tubules (tubule regions). To evaluate the proposed method, we calculate the tubule percentage (TP), i.e., the ratio of the tubule area to the total glandular area for 353 H and E images of the three TSs, and plot the distribution of these TP values. This plot shows the clear separation among these three scores, suggesting that the proposed algorithm is useful in distinguishing images of these TSs.

Citation Download Citation

Kien Nguyen, Michael Barnes, Chukka Srinivas, and Christophe Chefd'hotel "Automatic glandular and tubule region segmentation in histological grading of breast cancer", Proc. SPIE 9420, Medical Imaging 2015: Digital Pathology, 94200G (19 March 2015); https://doi.org/10.1117/12.2082322

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