A novel Kalman filter based video image processing scheme for two-photon fluorescence microscopy

Wenqing Sun; Xia Huang; Chunqiang Li; Chuan Xiao; Wei Qian

doi:10.1117/12.2216129

29 March 2016 A novel Kalman filter based video image processing scheme for two-photon fluorescence microscopy

Wenqing Sun, Xia Huang, Chunqiang Li, Chuan Xiao, Wei Qian

Proceedings Volume 9788, Medical Imaging 2016: Biomedical Applications in Molecular, Structural, and Functional Imaging; 97881I (2016) https://doi.org/10.1117/12.2216129
Event: SPIE Medical Imaging, 2016, San Diego, California, United States

Abstract

Two-photon fluorescence microscopy (TPFM) is a perfect optical imaging equipment to monitor the interaction between fast moving viruses and hosts. However, due to strong unavoidable background noises from the culture, videos obtained by this technique are too noisy to elaborate this fast infection process without video image processing. In this study, we developed a novel scheme to eliminate background noises, recover background bacteria images and improve video qualities. In our scheme, we modified and implemented the following methods for both host and virus videos: correlation method, round identification method, tree-structured nonlinear filters, Kalman filters, and cell tracking method. After these procedures, most of noises were eliminated and host images were recovered with their moving directions and speed highlighted in the videos. From the analysis of the processed videos, 93% bacteria and 98% viruses were correctly detected in each frame on average.

Citation Download Citation

Wenqing Sun, Xia Huang, Chunqiang Li, Chuan Xiao, and Wei Qian "A novel Kalman filter based video image processing scheme for two-photon fluorescence microscopy", Proc. SPIE 9788, Medical Imaging 2016: Biomedical Applications in Molecular, Structural, and Functional Imaging, 97881I (29 March 2016); https://doi.org/10.1117/12.2216129

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KEYWORDS

Video

Video processing

Bacteria

Image processing

Filtering (signal processing)

Viruses

Luminescence

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