Deformable membrane for the segmentation of cytological samples

Volker H. Metzler; Joerg Bredno; Thomas Martin Lehmann; Klaus Spitzer

doi:10.1117/12.310852

24 June 1998 Deformable membrane for the segmentation of cytological samples

Volker H. Metzler, Joerg Bredno, Thomas Martin Lehmann, Klaus Spitzer

Proceedings Volume 3338, Medical Imaging 1998: Image Processing; (1998) https://doi.org/10.1117/12.310852
Event: Medical Imaging '98, 1998, San Diego, CA, United States

Abstract

In clinical cytology quantitative parameters have to be extracted from a large number of biological samples to obtain diagnostically relevant and reproducible information. Computer-assisted microscopy can provide methods that increase the quality and comparability of clinical studies by reducing the subjective influence of human operators on their results. In order to guarantee the correctness of extracted parameters automatic and reliable segmentation of the samples is required. For the detection of cytological objects a novel deformable membrane model is presented which is strictly based on macroscopical mechanics and statics. This is appropriate for modeling physiological membranes, because their shape is determined exclusively by mechanical forces. The self-driven membrane converges iteratively towards a stable state, where the contrary forces are in balance. However, active contours may not yield sufficient detection quality for acquisition of quantitative parameters. Therefore, after convergence a stochastic optimization process corrects the contour according to local graylevel information. This yields a contour that is well- adapted to the local graylevel structure. Additionally, for subsequent cytometric quantifications a local measure of confidence is provided for the contour. this can be used to enhance the robustness of the extracted parameters by incorporating the confidence factors in the quantification process. The method is applied to cytological and histological samples of different magnification.

Citation Download Citation

Volker H. Metzler, Joerg Bredno, Thomas Martin Lehmann, and Klaus Spitzer "Deformable membrane for the segmentation of cytological samples", Proc. SPIE 3338, Medical Imaging 1998: Image Processing, (24 June 1998); https://doi.org/10.1117/12.310852

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