Optical biosensor for the detection of low concentrations of hydrogen peroxide in milk samples

Helena Vasconcelos; Ana Beatriz Matias Teixeira; João Mendes; João Araújo; Bernardo Dias; Pedro A. S. Jorge; Cristina M. Saraiva; Luis C. C. Coelho; José Manuel M. M. de Almeida

doi:10.1117/12.2621552

17 May 2022 Optical biosensor for the detection of low concentrations of hydrogen peroxide in milk samples

Helena Vasconcelos, Ana Beatriz Matias Teixeira, João Mendes, João Araújo, Bernardo Dias, Pedro A. S. Jorge, Cristina M. Saraiva, Luis C. C. Coelho, José Manuel M. M. de Almeida

Author Affiliations +

Proceedings Volume 12139, Optical Sensing and Detection VII; 121390W (2022) https://doi.org/10.1117/12.2621552
Event: SPIE Photonics Europe, 2022, Strasbourg, France

Abstract

A strategy for the detection of H₂O₂ as a milk adulterant using a single shot membrane sensor, is presented. Direct quantitative evaluation of H₂O₂ in raw, skimmed, semi-skimmed and whole milk was carried out based on a chemiluminescence reaction with luminol. For H₂O₂ water solutions a linear response was attained from 0.0001% to 0.007 %w/w, with a limit of detection of 3×10^-5 %w/w. A coefficient of determination, R² , greater than 0.97 was achieved, with a relative standard deviation (RSD) not exceeding 10%. In the analyzed milk samples, the lowest H₂O₂ concentration detected was 0.001%w/w for raw and for skim milk and 0.002%w/w for, semi-skimmed and whole milk. The presented method is original, sensitive, rapid, and cost-effective. Due to the achieved sensitivity the method has great potential to be used for H₂O₂ detection in diverse areas, such as environmental monitoring and food quality.

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Helena Vasconcelos, Ana Beatriz Matias Teixeira, João Mendes, João Araújo, Bernardo Dias, Pedro A. S. Jorge, Cristina M. Saraiva, Luis C. C. Coelho, and José Manuel M. M. de Almeida "Optical biosensor for the detection of low concentrations of hydrogen peroxide in milk samples", Proc. SPIE 12139, Optical Sensing and Detection VII, 121390W (17 May 2022); https://doi.org/10.1117/12.2621552

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