ABSTRACT
AIM To study the effect of total flavones of Clematis Filamentosa Dunn(TFCD) on adjusting autophagy against myocardial ischemia-reperfusion injury (MIRI). METHODS Forty male SD rats were divided randomly into five groups(n=8): sham group, model group(I/R), TFCD treatment group(TFCD), autophagy activator rapamycin treatment group(Rap) and TFCD+ rapamycin treatment group(TFCD+Rap). While Myocardial ischemia-reperfusion injury model was established by ligation of left anterior descending coronary artery (LAD) in rats. Hemodynamic indexes of heart were recorded, myocardial infarction area was observed, and serum lactate dehydrogenase (LDH), creatine kinase isoenzyme (CK-MB), total antioxidant capacity (T-AOC), nitric oxide (NO), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) were measured. The content of Caspase-3 and the activity of Caspase-3 were measured. The protein expression of autophagy associated genes LC3-II and Beclin1 in myocardium was detected by using RT-PCR and Western blot. RESULTS TFCD treatment remarkably improved hemodynamics function, reduced myocardial infarct size, enhanced the contents of T-AOC, NO and eNOS, decreased the contents of LDH, CK-MB, iNOS and the activity level of caspase-3(P<0.05 or P<0.01). In addition, TFCD treatment significantly inhibited the expression of autophagy-related proteins LC3-II and Beclin-1. Autophagy activator inhibited the effect of TFCD. CONCLUSION The cardioprotection of TFCD against myocardial ischemia reperfusion injury may be mediated through improving antioxidant, regulating NO, reducing apoptosis and inhibiting autophagy.
- Mizushima N, Komatsu M. Autophagy: renovation of cells and tissues[J]. Cell, 2011, 147(4): 728--741.Google ScholarCross Ref
- Green D R, Levine B. To be or not to be? How selective autophagy and cell death govern cell fate[J]. Cell, 2014, 157(1): 65--75.Google ScholarCross Ref
- Wu D, Zhang K, Hu P. The role of autophagy in acute myocardial infarction[J]. Front Pharmacol, 2019, 10: 551.Google ScholarCross Ref
- Binder A, Ali A, Chawla R, et al. Myocardial protection from ischemia-reperfusion injury post coronary revascularization[J]. Expert Rev Cardiovasc Ther, 2015, 13( 9): 1045--1057.Google ScholarCross Ref
- Schmidt M R, Pryds K, Botker H E. Novel adjunctive treatments of myocardial infarction[J]. World J Cardiol, 2014, 6( 6): 434--443.Google ScholarCross Ref
- Yin Z, Pascual C, Klionsky D. Autophagy: machinery and regulation[J]. Microbial Cell, 2016, 3(12): 588--596.Google ScholarCross Ref
- Ma S, Wang Y, Chen Y, et al. The role of the autophagy in myocardial ischemia/reperfusion injury[J]. BBA-Biomembranes, 2015, 1852(2): 271--276.Google Scholar
- Nie Y, Chen X Y, Yang Y J, et al. The protective effect of the Flavones from Clematis filamentosa Dunn on myocardial ischemia[J]. Chin J Exp Tradit Med Form, 2014, 20(22): 176--179.Google Scholar
- Nie Y, Hang H C, Chen X Y, et al. Protective Effect of Total Flavones in Clematis filamentosa Dunn on Myocardial Ischemia-Reperfusion Injury in Rats[J]. Chin Tradit Pat Med, 2016, 38(12): 2677--2679.Google Scholar
- Nie Y, Li B, Zhu J F, et al. Protective Effect of Total Flavones in Clematis filamentosa Dunn on Myocardial Cells with Hypoxia/Reoxygenation induced Injury in Neonatal Rats[J]. Chin Arch Tradit Chin Med, 2017, 35(7): 1876--1878.Google Scholar
- Nie Y, Ding L, Huang H C, et al. Effects of PI3K/Akt-eNOS signaling pathway on total flavones in Clematis filamentosa post-conditioning alleviated of myocardial ischemia-reperfusion injury in rats[J]. Chin J Chin Mate Med, 2018, 43(23): 4692--4697.Google Scholar
- Heusch G, Gersh BJ. The pathophysiology of acute myocardial infarction and strategies of protection beyond reperfusion: acontinual challenge[J]. Eur Heart J, 2017, 38(11): 774--784.Google Scholar
- Hurtley S M. Mitochondrial quality control[J]. Science, 2015, 350(6264): 1052--1053.Google ScholarCross Ref
- Shirakabe A, Zhai P, Ikeda Y, et al. Drp1-dependent mitochondrial autophagy plays a protective role against pressure overload-induced mitochondrial dysfunction and heart failure[J]. Circulation, 2016, 133(13): 1249--1263.Google ScholarCross Ref
- Zheng Y, Shi B, Ma M, et al. The novel relationship between Sirt3 and autophagy in myocardial ischemia-reperfusion[J]. J Cell Physiol, 2019, 234(5): 5488--5495.Google ScholarCross Ref
- Sarkar S, Korolchuk VI, Renna M, et al. Complex inhibitory effects of nitric oxide on autophagy[J]. Mol Cell. 2011;43(1): 19--32.Google Scholar
- De Andrés M C, Galdo F, Blanco F J. 222 Nitric oxide(NO) in some conditions induces autophagy in human articular chondrocytes[J]. Osteoarthr Cartilage, 2008, 16.Google Scholar
- He H, Feng Y S, Zang L, et al.. Nitric oxide induces apoptosis and autophagy; autophagy down-regulates NO synthesis in physalin A-treated A375-S2 human melanoma cells[J]. Food Chem Toxicol, 2014, 71.Google Scholar
- Narne P, Pandey V, Phanithi PB. Role of Nitric Oxide and Hydrogen Sulfide in Ischemic Stroke and the Emergent Epigenetic Underpinnings[J]. Mol Neurobiol. 2019;56(3): 1749--1769.Google Scholar
- Czarnowska E, Kurzelewski M, Beresewicz A. The role of endogenous nitric oxide in inhibition of ischemia/reperfusion-induced cardiomyocyte apoptosis[J]. Folia Histochem Cytobiol, 2001; 39(2): 179--80.Google Scholar
- Dong Y, Undyala V V, Gottlieb RA, et al. Autophagy: definition, molecular machinery and potential role in myocardial ischemia reperfusion injury[J]. J Cardiovasc Pharmacol Ther, 2010, 15(3): 220--230.Google ScholarCross Ref
- Bialik S, Dasari S K, Kimchi A. Autophagy-dependent cell death where, how and why a cell eats itself to death[J]. J Cell Sci, 2018, 131(18):215152.Google ScholarCross Ref
- Yang W J, Duan Q J, Zhu X, et al. Follistatin-like 1 attenuates ischemia/reperfusion injury in cardiomyocytes via regulation of autophagy[J]. Biomed Res Int, 2019: 9537382.Google Scholar
- Klionsky D J, Abdalla F C, Abeliovich H, et al. Guidelines for the use and interpretation of assays for monitoring autophagy[J]. Autophagy, 2012, 8(4): 445--544.Google ScholarCross Ref
- Carroll B, Otten E G, Manni D, et al. Oxidation of SQSTM1/p62 mediates the link between redox state and protein homeostasis[J]. Nat Commun, 2018, 9(1): 1--11.Google ScholarCross Ref
- Qing Zhong, Huifang Chen etc. Dual-function antibacterial micelle via self-assembling block copolymers with various antibacterial nanoparticles[J]. acs omega.2020.5: 8523--8533.Google Scholar
- Huifang Chen. Does School Enterprise Cooperation to Promote Students' Occupation Career Planning: on the Inspirational Experience of School-enterprise Cooperation from Developed Countries[J]. Review of Global Academics, Vol. 1 No. 2 Aug. 2014: 56--72.Google Scholar
- Huifang Chen. etc. Application of chitosan in drug controlled release microcapsules[J]. Chinese Journal of Integrative Medicine.2019, 8(8): 135--137.Google Scholar
- Huifang Chen. etc. Exploration on teaching reform of "Applied Microbiology and Immunology" course for medical specialty [J]. Journal of aviation military medicine, 2016, 8(8): 123--125.Google Scholar
- Huifang Chen. etc. Research on teaching reform of biopharmaceutical technology[J]. Chinese Journal of misdiagnosis. 2018, 5(8): 182--184.Google Scholar
Index Terms
- Effect of Total Flavones in Clematis Filamentosa Dunn against Myocardial Ischemia Reperfusion Injury by Adjusting Autophagy in Rats
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