A novel conjugate unitary ESPRIT (CU-ESPRIT) algorithm for the joint direction of departure (DOD), and direction of arrival (DOA), estimation in a bistatic MIMO radar is proposed. A new virtual array is formed by using the properties of noncircular signals, and the properties of the centro-Hermitian matrix are employed to convert the complex-valued data matrix into a real-valued data matrix. Then the real-valued rotational invariance properties of the new virtual array are determined to estimate DODs and DOAs, which are paired automatically. The proposed method provides better angle estimation performance and detects more targets owing to double number of MIMO virtual array elements. Simulation results are presented to verify the effectiveness of the proposed algorithm.