Low Voltage Pulse Application to Biological Cells

Hidenori OTSUKA; Saya OKIMURA; Masako NAGAMURA; Daisuke MATSUKUMA; Koichi KUTSUZAWA; Naoki MATSUDA; Hirotaka OKABE

doi:10.1587/transele.E96.C.348

Special Section on Recent Progress in Molecular and Organic Devices

Low Voltage Pulse Application to Biological Cells

Hidenori OTSUKA, Saya OKIMURA, Masako NAGAMURA, Daisuke MATSUKUMA, Koichi KUTSUZAWA, Naoki MATSUDA, Hirotaka OKABE

Author information

Keywords: pulsed electric fields (PEF), low voltage pulse, layer by layer (LBL), cell viability

JOURNAL RESTRICTED ACCESS

2013 Volume E96.C Issue 3 Pages 348-352

DOI https://doi.org/10.1587/transele.E96.C.348

Details

Abstract

As an application of low electric field to biomedical engineering, this paper attempts to study the dose-effect of biological effects caused by msPEF with experiments on HeLa cells. MTT assay was used to trace the cell electroporation and examine cell viability. It is observed that with the increasing electric field intensity and pulse numbers, IRE effects will occur successively.

Corresponding author

Register with J-STAGE for free!