Vehicle routing is an important combinatorial optimization problem. In real transport networks,the travel speed and travel time of roads have large time-variability and randomness. The study of vehicle routing problem in time-dependent network has even more practical value than static network VRP problem. This paper combines the features of time-dependent networks and gives the mathematical models of the time-dependent vehicle routing problem. On this basis, the traditional ant colony optimization algorithm is improved. A new path transfer strategy of ants and new dynamic pheromone update strategy applicable to time-dependent network are proposed. Based on these strategies, the improved ant colony algorithm is given for solving the vehicle routing problem in time-dependent networks. The simulation results show that the algorithm can effectively solve the vehicle routing problem in time-dependent network and has better computational efficiency and convergence speed.