To enhance the throughput while satisfying the quality of service (QoS) requirements of wireless local area networks (WLANs), this paper proposes a distributed coordination function-based (DCF-based) medium access control (MAC) protocol that realizes centralized radio resource management (RRM) for a basic service set. In the proposed protocol, an access point (AP) acts as a master to organize the associated stations and attempts to reserve the radio resource in a conventional DCF-manner. Once the radio resource is successfully reserved, the AP controls the access of each station by an orthogonal frequency division multiple access (OFDMA) scheme. Because the AP assigns radio resources to the stations through the opportunistic two-dimensional scheduling based on the QoS requirements and the channel condition of each station, the transmission opportunities can be granted to the appropriate stations. In order to reduce the signaling overhead caused by centralized RRM, the proposed protocol introduces a station-grouping scheme which groups the associated stations into clusters. Moreover, this paper proposes a heuristic resource allocation algorithm designed for the DCF-based MAC protocol. Numerical results confirm that the proposed protocol enhances the throughput of WLANs while satisfying the QoS requirements with high probability.