In situ Observation of Immobilization of Cytochrome c into Hydrophobic DNA Nano-Film

Naoki MATSUDA; Hirotaka OKABE; Ayako OMURA; Miki NAKANO; Koji MIYAKE; Toshihiko NAGAMURA; Hideki KAWAI

doi:10.1587/transele.2018FUS0004

Special Section on Fundamentals and Applications of Advanced Semiconductor Devices

In situ Observation of Immobilization of Cytochrome c into Hydrophobic DNA Nano-Film

Naoki MATSUDA, Hirotaka OKABE, Ayako OMURA, Miki NAKANO, Koji MIYAKE, Toshihiko NAGAMURA, Hideki KAWAI

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Keywords: Hydrophobic DNA nano-film, cytochrome c, immobilization, slab optical waveguide spectroscopy

JOURNAL RESTRICTED ACCESS

2019 Volume E102.C Issue 6 Pages 471-474

DOI https://doi.org/10.1587/transele.2018FUS0004

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Abstract

Hydrophobic DNA (H-DNA) nano-film was formed as the surface modifier on a thin glass plate working as a slab optical waveguide (SOWF). Cytochrom c (cytc) molecules were immobilized from aqueous solution with direct contacting to the H-DNA nano-film for 30 minutes. From SOWG absorption spectral changes during automated solution exchange (SE) processes, it was found that about 28.1% of cytc molecules was immobilized in the H-DNA nano-film with keeping their reduction functionality by reducing reagent.

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