In situ Observation of Capturing BTB Molecules from Aqueous Solutions with Hydrophobic DNA Nano-Film

Naoki MATSUDA; Hirotaka OKABE; Ayako OMURA; Miki NAKANO; Koji MIYAKE; Toshihiko NAGAMURA; Hideki KAWAI

doi:10.1587/transele.2018OMS0018

Special Section on Recent Progress in Organic Molecular Electronics and Biotechnology

In situ Observation of Capturing BTB Molecules from Aqueous Solutions with Hydrophobic DNA Nano-Film

Naoki MATSUDA, Hirotaka OKABE, Ayako OMURA, Miki NAKANO, Koji MIYAKE, Toshihiko NAGAMURA, Hideki KAWAI

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Keywords: hydrophobic DNA nano-film, bromothymol blue, immobilization, slab optical waveguide spectroscopy, in situ observation

JOURNAL RESTRICTED ACCESS

2019 Volume E102.C Issue 2 Pages 203-206

DOI https://doi.org/10.1587/transele.2018OMS0018

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Abstract

Hydrophobic DNA (H-DNA) nano-film was formed on a thin glass plate of 50μm thick working as a slab optical waveguide. Bromothymol blue (BTB) molecules were immobilized from aqueous solution with direct contacting to the H-DNA nano-film for 20 minutes. From changes in absorption spectra observed with slab optical wave guide (SOWG) during automated solution exchange (SE) processes for 100 times, it was found that about 95% of bromothymol blue (BTB) molecules was immobilized in the H-DNA nano-film with keeping their functionality of color change responsible to pH change in the solution.

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