Building of HD MACs Using Cell Processing Robot for   Cartilage Regeneration

Takeshi Shimoto; Koichi Nakayama; Shuichi Matsuda; Yukihide Iwamoto

doi:10.20965/jrm.2012.p0347

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JRM Vol.24 No.2 pp. 347-353

doi: 10.20965/jrm.2012.p0347

(2012)

Paper:

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Building of HD MACs Using Cell Processing Robot for Cartilage Regeneration

Takeshi Shimoto^, Koichi Nakayama^, Shuichi Matsuda^,
and Yukihide Iwamoto^***

^*Faculty of Engineering, Kyushu Sangyo University, 2-3-1 Matsukadai, Higashi-ku, Fukuoka 813-8503, Japan

^**Faculty of Science and Engineering, Saga University, 1 Honjo-machi, Saga 840-8502, Japan

^***Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan

Received:

September 30, 2011

Accepted:

January 26, 2012

Published:

April 20, 2012

Keywords:

cells construct, rapid prototyping system, mesenchymal stem cell

Abstract

We have been working on the development of scaffold free cell delivery system, and showing good results in the regeneration of rabbit osteochondral defect for more than three years by implantation of molded mesenchymal stem cells construct without use of exogenous factors. The entire procedure is simple, yet it takes time and labor to build a single construct by even experienced hands. Therefore, this study was aimed to develop a cell processing robot for building scaffoldfree HD MACs (High-Density Mesenchymal stem cell Autologous Constructs) for cartilage regeneration. As a result, the scaffold-free HD MACs could be obtained with manual procedure just as the same without contamination. We have implanted the construct in a rabbit with osteochondral defect in the knee. In this study, we have developed a cell processing robot for cartilage regeneration. The entire system is almost GMP ready. We are preparing clinical trials at Kyushu University Hospital for HD MACs implantation to cartilage defect with this robot system.

Cite this article as:

T. Shimoto, K. Nakayama, S. Matsuda, and Y. Iwamoto, “Building of HD MACs Using Cell Processing Robot for Cartilage Regeneration,” J. Robot. Mechatron., Vol.24 No.2, pp. 347-353, 2012.

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References

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This article is published under a Creative Commons Attribution-NoDerivatives 4.0 Internationa License.

[1] [1] M. Harimoto, M. Yamato, M. Hirose, C. Takahashi, Y. Isoi, A. Kikuchi, and T. Okano, “Novel approach for achieving doublelayered cell sheets co-culture: overlaying endothelial cell sheets onto monolayer hepatocytes utilizing temperature-responsive culture dishes,” J. Biomedical Materials Res., Vol.62, No.3, pp. 464-470, 2002.

[2] [2] M. Nakamura, A. Kobayashi, F. Takagi, A. Watanabe, Y. Hiruma, K. Ohuchi, Y. Iwasaki, M. Horie, I. Morita, and S. Takatani, “Biocompatible inkjet printing technique for designed seeding of individual living cells,” Tissue engineering, Vol.11, No.11-12, pp. 1658-1666, 2005.

[3] [3] V. L. Tsang, A. A. Chen, L. M. Cho, K. D. Jadin, R. L. Sah, S. De-Long, J. L. West, and S. N. Bhatia, “Fabrication of 3D hepatic tissues by additive photopatterning of cellular hydrogels,” The FASEB Journal, Vol.21, No.3, pp. 790-801, 2006.

[4] [4] A. P. Rago, D. M. Dean, and J. R. Morgan, “Controlling cell position in complex heterotypic 3D microtissues by tissue fusion,” Biotechnology and Bioengineering, Vol.102, No.4, pp. 1231-1241, 2009.

[5] [5] E. D. Miller, G. W. Fisher, L. E. Weiss, L. M. Walker, and P. G. Campbell, “Dose-dependent cell growth in response to concentration modulated patterns of FGF-2 printed on fibrin,” Biomaterials, Vol.27, No.10, pp. 2213-2221, 2006.

[6] [6] C. Norotte, F. S. Marga, L. E. Niklason, and G. Forgace, “Scaffold-Free Vascular Tissue Engineering Using Bioprinting,” Biomaterials, Vol.30, No.30, pp. 5910-5917, 2009.

[7] [7] M. Ochi, N. Adachi, H. Nobuto, S. Yanada, Y. Ito, and M. Agung, “Articular cartilage repair using tissue engineering technique – novel approach with minimally invasive procedure,” Artificial Orqans, Vol.28, No.1, pp. 28-32, 2004.

[8] [8] E. B. Hunziker, I. M. Driesang, and C. Saager, “Structural barrier principle for growth factor-based articular cartilage repair,” Clinical Orthopaedics and Related Research, Vol.391, pp. 182-189, 2001.

[9] [9] P. Mainil-Varlet, F. Rieser, S. Grogan, W. Mueller, C. Saager, and R. P. Jakob, “Articular cartilage repair using a tissue-engineered cartilage-like implant: an animal study,” Osteoarthritis and Cartilage, Vol.9, pp. 6-15, 2001.

Building of HD MACs Using Cell Processing Robot for Cartilage Regeneration

Takeshi Shimoto*, Koichi Nakayama**, Shuichi Matsuda***, and Yukihide Iwamoto***

Takeshi Shimoto^, Koichi Nakayama^, Shuichi Matsuda^,
and Yukihide Iwamoto^***