Authors:
Cristina Martínez-Gómez
1
;
2
;
Alberto Olmo
1
;
2
;
Gloria Huertas
1
;
2
;
Pablo Pérez
1
;
2
;
Andres Maldonado-Jacobi
1
;
2
and
Alberto Yúfera
1
;
2
Affiliations:
1
CSIC- Universidad de Sevilla, Spain
;
2
Universidad de Sevilla, Spain
Keyword(s):
ECIS, Bioimpedance, Cell Culture, Cell Location, Cell Motility, Brownian Movement, CMOS.
Abstract:
This work proposes a method for studying and monitoring in real-time a single cell on a 2D electrode matrix, of great interest in cell motility assays and in the characterization of cancer cell metastasis. A CMOS system proposal for cell location based on occupation maps data generated from Electrical Cell-substrate Impedance Spectroscopy (ECIS) has been developed. From this cell model, obtained from experimental assays data, an algorithm based on analysis of the 8 nearest neighbors has been implemented, allowing the evaluation of the cell center of mass. The path followed by a cell, proposing a Brownian route, has been simulated with the proposed algorithm. The presented results show the success of the approach, with accuracy over 95% in the determination of any coordinate (x, y) from the expected center of mass.