Authors:
Juan A. Serrano
1
;
Pablo Pérez
1
;
Andrés Maldonado
1
;
María Martín
2
;
Alberto Olmo
1
;
Paula Daza
2
;
Gloria Huertas
1
and
Alberto Yúfera
1
Affiliations:
1
Instituto de Microelectrónica de Sevilla (IMSE), Universidad de Sevilla, Spain
;
2
Facultad de Biología, Universidad de Sevilla, Spain
Keyword(s):
Biomedical Circuits, Impedance Spectroscopy, Bioimpedance, Electrode-model, Oscillation based Test Circuits (OBT), ECIS.
Abstract:
This paper presents the fitting process followed to adjust the parameters of the electrical model associated to a cell-electrode system in Electrical Cell-substrate Impedance Spectroscopy (ECIS) technique, to the experimental results from cell-culture assays. A new parameter matching procedure is proposed, under the basis of both, mismatching between electrodes and time-evolution observed in the system response, as consequence of electrode fabrication processes and electrochemical performance of electrode-solution interface, respectively. The obtained results agree with experimental performance, and enable the evaluation of the cell number in a culture, by using the electrical measurements observed at the oscillation parameters in the test circuits employed.