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Authors: Arban Uka ; Gent Imeraj ; Bjorna Qesaraku and Besmir Shehu

Affiliation: Department of Computer Engineering, Epoka University, Tirane, Albania

Keyword(s): Microscopy, Fourier Ptychography, Refocusing, Microfluidic Chambers.

Abstract: Fourier ptychography is a computational imaging technique that is used to overcome the physical limitations in determining the spatial resolution of an optical system by combining a large number of low resolution images. The low resolution images are acquired using programmed illumination from an array of light sources, thus enabling the scanning of the k-space, which is the reciprocal space of the real domain. The use of this approach would improve the resolution when biological samples from patients are analyzed in multiparametric chambers. When an off-center light source is used at an oblique incidence angle, the optical path length changes thus defocusing the expected image. The common depth of focus of microscopes is a few micrometers and when a chamber of thickness from 0.5-1.0 cm is used, an adjustment of the focusing is needed. Here in this work, we report Fourier ptychography using LED illumination and an improved image quality is acquired when refocusing is implemented.

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Paper citation in several formats:
Uka, A.; Imeraj, G.; Qesaraku, B. and Shehu, B. (2022). Fourier Ptychography Microscopy Resolution Improvement Employing Refocusing. In Proceedings of the 15th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2022) - BIODEVICES; ISBN 978-989-758-552-4; ISSN 2184-4305, SciTePress, pages 191-195. DOI: 10.5220/0010915500003123

@conference{biodevices22,
author={Arban Uka. and Gent Imeraj. and Bjorna Qesaraku. and Besmir Shehu.},
title={Fourier Ptychography Microscopy Resolution Improvement Employing Refocusing},
booktitle={Proceedings of the 15th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2022) - BIODEVICES},
year={2022},
pages={191-195},
publisher={SciTePress},
organization={INSTICC},
doi={10.5220/0010915500003123},
isbn={978-989-758-552-4},
issn={2184-4305},
}

TY - CONF

JO - Proceedings of the 15th International Joint Conference on Biomedical Engineering Systems and Technologies (BIOSTEC 2022) - BIODEVICES
TI - Fourier Ptychography Microscopy Resolution Improvement Employing Refocusing
SN - 978-989-758-552-4
IS - 2184-4305
AU - Uka, A.
AU - Imeraj, G.
AU - Qesaraku, B.
AU - Shehu, B.
PY - 2022
SP - 191
EP - 195
DO - 10.5220/0010915500003123
PB - SciTePress

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