Abstract
Thin-Layer Chromatography images are used to detect and identify the presence of specific oligosaccharides, expressed by the existence, at different positions, of bands in the gel image. 1D gaussian deconvolution, commonly used for band detection, does not produce good results due to the large curvature observed in the bands. To overcome this uncertainty on the band position, we propose a novel feature extraction methodology that allows an accurate modeling of curved bands. The features are used to classify the data into two different classes, to differentiate normal from pathologic cases. The paper presents the developed methodology together with the analysis and discussion of the results.
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Bajla, I., Holländer, I., Burg, K.: Improvement of Electrophoretic Gel Image Analysis - M. S. R. 1(1) (2001)
Ye, X., Suen, C., Cheriet, M., Wang, E.: A Recent Development in Image Analysis of Electrophoresis Gels - Vision Interface 1999, Canada (1999)
Shadle, S., Allen, D., Guo, H., Pogozelski, W., Bashkin, J., Tullius, T.: Quantitative analysis of electrophoresis data: novel curve fitting methodology and its application to the determination of a protein-DNA binding constant. Nucleic Acids Research 25(4) (1997)
Sousa, A., Aguiar, R., Mendonça, A.M., Campilho, A.: Automatic Lane and Band Detection in Images of Thin Layer Chromatography. In: Campilho, A.C., Kamel, M.S. (eds.) ICIAR 2004. LNCS, vol. 3212, pp. 372–380. Springer, Heidelberg (2004)
Heijden, F., Duin, R., Ridder, D., Tax, D.: Classification, Parameter Estimation and State Estimation. John Wiley and Sons, England (2004)
Sewell, A.: Urinary Oligosaccharides. In: Techniques in Diagnostic Human Biochemical Genetics, pp. 219–231. Willey-Liss, Inc. (1991)
Thomas, G.: Disorders of Glycoprotein Degradation. a-Mannosidosis, b-Mannosidosis, Fucosidosis and Sialidosis. In: Disorders of Glycoprotein Degradation, ch. 140
Durant, G., Seta, N.: Protein Glycosylation and Diseases: Blood and Urinary Oligosaccharides as Markers for Diagnosis and Therapeutic Monitoring. Clinical Chemistry 46(6), 795–805 (2000)
Marques de Sá, J.: Applied Statistics using SPSS, STATISTICA and MATLAB. Springer, Heidelberg (2003)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2005 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Sousa, A.V., Mendonça, A.M., Campilho, A., Aguiar, R., Miranda, C.S. (2005). Feature Extraction for Classification of Thin-Layer Chromatography Images. In: Kamel, M., Campilho, A. (eds) Image Analysis and Recognition. ICIAR 2005. Lecture Notes in Computer Science, vol 3656. Springer, Berlin, Heidelberg. https://doi.org/10.1007/11559573_118
Download citation
DOI: https://doi.org/10.1007/11559573_118
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-29069-8
Online ISBN: 978-3-540-31938-2
eBook Packages: Computer ScienceComputer Science (R0)