Abstract
DNA sequencing is an important technology for the determination of the sequences of nucleotides that make up a given DNA fragment. In view of the limitations of current sequencing technology, it would be advantageous to have a DNA sequencing method that provides the sequences of long DNA fragments and is amenable to automation. Sequencing by Hybridization (SBH) is a challenging alternative to the classical sequencing methods. The basic approach is to build an array (Sequencing Chip) of short DNA fragments of lenght l and to use biochemical methods for finding all substrings of lenght l of an unknown DNA fragment. Combinatorial algorithms are then used to reconstruct the sequence of the fragment from the l-tuple composition. In this article we review biochemical, mathematical, and technological aspects of SBH and present a new sequencing chip design which might allow significant chip miniaturization without loss of the resolution of the method.
The research was supported in part by the National Science Foundation under the grant CCR-9308567 and by the National Institutes of Health under the grant 1R01 HG00987-01
The research was supported in part by the National Institutes of Health under the grant HG-00813 and by the Department of Energy under the grant DE-FG03-92-ER81275
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Pevzner, P.A., Lipshutz, R.J. (1994). Towards DNA sequencing chips. In: Prívara, I., Rovan, B., Ruzička, P. (eds) Mathematical Foundations of Computer Science 1994. MFCS 1994. Lecture Notes in Computer Science, vol 841. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-58338-6_64
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DOI: https://doi.org/10.1007/3-540-58338-6_64
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