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In Vivo Quantitative Proteome Profiling: Planning and Evaluation of SILAC Experiments

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Quantitative Methods in Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 893))

Abstract

Mass spectrometry-based quantitative proteomics can identify and quantify thousands of proteins in complex biological samples. Improved instrumentation, quantification strategies and data analysis tools now enable protein analysis on a genome-wide scale. Particularly, quantification based on stable isotope labeling with amino acids (SILAC) has emerged as a robust, reliable and simple method for accurate large-scale protein quantification. The spectrum of applications ranges from bacteria and eukaryotic cell culture systems to multicellular organisms. Here, we provide a step-by-step protocol on how to plan and perform large-scale quantitative proteome analysis using SILAC, from sample preparation to final data analysis.

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Author information

Authors and Affiliations

  1. Cell Signalling and Mass Spectrometry Group, Max Delbrueck Center for Molecular Medicine, Berlin, Germany

    Marieluise Kirchner & Matthias Selbach

Authors
  1. Marieluise Kirchner
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  2. Matthias Selbach
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Corresponding author

Correspondence to Matthias Selbach .

Editor information

Editors and Affiliations

  1. Fak. Medizin, Medizinische Proteom Center, Universität Bochum, Universitätsstraße 150, Bochum, 44801, Germany

    Katrin Marcus

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Kirchner, M., Selbach, M. (2012). In Vivo Quantitative Proteome Profiling: Planning and Evaluation of SILAC Experiments. In: Marcus, K. (eds) Quantitative Methods in Proteomics. Methods in Molecular Biology, vol 893. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-61779-885-6_13

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  • DOI: https://doi.org/10.1007/978-1-61779-885-6_13

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-61779-884-9

  • Online ISBN: 978-1-61779-885-6

  • eBook Packages: Springer Protocols

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