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A Novel Lab-on-Disk System for Pathogen Nucleic Acids Analysis in Infectious Diseases

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Sensors (CNS 2018)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 539))

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Abstract

The miniaturization of Real Time PCR (qPCR) systems is a crucial point towards the development of “genetic point-of-care” (PoC) that are able to offer sample-in-answer-out diagnostic analysis. Centralized laboratories and specialized staffs are needed for conventional DNA analysis. To solve this issue, we propose an innovative easy-to-use PoC technology based on a Lab-on-Disk miniaturized system, integrating nucleic acids extraction process based on Mags-Beads technology and detection based on qPCR. Lab-on-Disk system is composed by a polycarbonate disk with reagent-on-board for DNA extraction and a qPCR silicon-chip. A customized reader integrating electronic and optical modules was developed for driving the polycarbonate disk. Here we present results in the detection of Hepatitis B Virus (HBV) genome.

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Acknowledgements

The authors acknowledge HSG-IMIT for the disk design development and CLONIT for providing HBV sample for the extraction and detection tests.

Author information

Authors and Affiliations

  1. Dipartimento di Fisica ed Astronomia, Università degli Studi di Catania, Catania, Italy

    Emanuele Luigi Sciuto

  2. STMicroelectronics, Stradale Primosole 50, Catania, Italy

    Salvatore Petralia & Sabrina Conoci

Authors
  1. Emanuele Luigi Sciuto
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  2. Salvatore Petralia
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  3. Sabrina Conoci
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Corresponding authors

Correspondence to Emanuele Luigi Sciuto or Salvatore Petralia .

Editor information

Editors and Affiliations

  1. University of Catania, Catania, Italy

    Bruno Andò

  2. IFAC-CNR, Sesto Fiorentino, Florence, Italy

    Francesco Baldini

  3. University of Rome Tor Vergata, Rome, Italy

    Corrado Di Natale

  4. Department of Information Engineering (DII), University of Brescia, Brescia, Italy

    Vittorio Ferrari

  5. University of Catania, Catania, Italy

    Vincenzo Marletta

  6. Department of Chemistry, University of Florence, Sesto Fiorentino, Florence, Italy

    Giovanna Marrazza

  7. University of Palermo, Palermo, Italy

    Valeria Militello

  8. University of Padova, Padua, Italy

    Giorgia Miolo

  9. Sapienza University of Rome, Rome, Italy

    Marco Rossi

  10. Università Politecnica delle Marche (UNIVPM), Ancona, Italy

    Lorenzo Scalise

  11. IMM-CNR, Lecce, Italy

    Pietro Siciliano

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Sciuto, E.L., Petralia, S., Conoci, S. (2019). A Novel Lab-on-Disk System for Pathogen Nucleic Acids Analysis in Infectious Diseases. In: Andò, B., et al. Sensors. CNS 2018. Lecture Notes in Electrical Engineering, vol 539. Springer, Cham. https://doi.org/10.1007/978-3-030-04324-7_16

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  • DOI: https://doi.org/10.1007/978-3-030-04324-7_16

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-030-04323-0

  • Online ISBN: 978-3-030-04324-7

  • eBook Packages: EngineeringEngineering (R0)

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