Abstract
A novel paper-based biosensors for the measurement of urinary phenylalanine (Phe) was developed and the experimental results here reported. The proposed biosensor is featured by a silicon part integrating temperature sensors and heaters and a polycarbonate ring to form a microchamber. The reagent-on-board format allows a fast and easy self-testing directly from patients. The biosensors is thermally driven by a customized instrument and software. The detection strategy employed is based on the specific reaction of Phenylalanine Ammonia Lyase enzyme to produce ammonia and trans-cinnamic acid from Phenylalanine. The increase of pH value is proportional to the Phe amount and can be monitored by the color changes of a dye solution. The proposed system is suitable to detect the phenylalanine levels in a linear dynamic range concentration from 20 to 3000 μM.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Kure, S., Hou, D.C., Ohura, T., et al.: Tetrahydrobiopterin-responsive phenylalanine hydroxylase deficiency. J. Pediatr. 135, 375–378 (1999)
Scriver, C.R., Kaufman, S.: Hyperphenylalaninemia: phenylalanine hydroxylase deficiency, 8th edn. Mc Graw-Hill (2001)
Fonnesbeck, C.J., McPheeters, M.I., et al.: Estimating the probability of IQ impairment from blood phenylalanine for phenylketonuria patients: a hierarchical meta-analysis. J. Inherited Metab. Dis. 36, 757–766 (2013)
Petralia, S., Conoci, S.: PCR technologies for point of care testing: progress and perspectives. ACS Sens. (2), 876–891 (2017)
Guarnaccia, M., Iemmolo, R., Petralia, S., Conoci, S., Cavallaro, S.: Miniaturized real-time PCR on a Q3 system for rapid KRAS genotyping. Sensors 17(831), 1–9 (2017)
Messina, M.A., Meli, C., Conoci, S., Petralia, S.: A facile method for urinary phenylalanine measurement on paper-based lab-on-chip for PKU therapy monitoring. Analyst 142, 4629–4632 (2017)
Petralia, S., Sciuto, E.L., Di Pietro, M.L., Zimbone, M., Grimaldi, M.G., Conoci, S.: Innovative chemical strategy for PCR-free genetic detection of pathogens by an integrated electrochemical biosensor. Analyst 142, 2090–2093 (2017)
Santangelo, M.F., Sciuto, E.L., Busacca, A.C., Petralia, S., Conoci, S., Libertino, S.: Si photomultipliers for bio-sensing applications. IEEE J. Sel. Top. Quantum Electron. 22(3) (2016)
Santangelo, M.F., Sciuto, E.L., Busacca, A.C., Petralia, S., Conoci, S., Libertino, S.: Si PM as miniaturised optical biosensor for DNA-microarray applications. Sens. BioSens. Res. (6) 95–98 (2015)
Author information
Authors and Affiliations
Corresponding authors
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2019 Springer Nature Switzerland AG
About this paper
Cite this paper
Messina, M.A., Raudino, F., Fiumara, A., Conoci, S., Petralia, S. (2019). A Novel Paper-Based Biosensor for Urinary Phenylalanine Measurement for PKU Therapy Monitoring. In: Andò, B., et al. Sensors. CNS 2018. Lecture Notes in Electrical Engineering, vol 539. Springer, Cham. https://doi.org/10.1007/978-3-030-04324-7_26
Download citation
DOI: https://doi.org/10.1007/978-3-030-04324-7_26
Published:
Publisher Name: Springer, Cham
Print ISBN: 978-3-030-04323-0
Online ISBN: 978-3-030-04324-7
eBook Packages: EngineeringEngineering (R0)