Abstract
In this study, we introduce a morphological analysis of segmented tumour cells from histopathology images concerning the recognition of cell overlapping. The main research problem considered is to distinguish how many cells are located in a structure, which is composed of overlapping cells. In our experiments, we used convolutional neural network models to provide recognition of the number of cells. For the medical data used: Ki-67 histopathology images, we achieved a high f1-score result. Therefore, our research proves the assumption to use convolutional neural networks for morphological analysis of segmented objects derived from medical images.
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References
Buiu, C., Dănăilă, V.-R., Răduţă, C.N.: MobileNetV2 ensemble for cervical precancerous lesions classification. Processes 8(5), 595 (2020)
Chetoui, M., Akhloufi, A.M.: Explainable diabetic retinopathy using EfficientNET. In: 42nd Annual International Conference of the IEEE Engineering in Medicine & Biology Society (EMBC). IEEE (2020)
Ebrahimi, A., Suhuai, L., Raymond, C.: Introducing transfer leaming to 3D ResNet-18 for Alzheimer’s disease detection on MRI Images. In: 35th International Conference on Image and Vision Computing New Zealand (IVCNZ). IEEE (2020)
Fouad, S., Landini, G., Randell, D., Galton, A.: Morphological separation of clustered nuclei in histological images. In: Campilho, A., Karray, F. (eds.) ICIAR 2016. LNCS, vol. 9730, pp. 599–607. Springer, Cham (2016). https://doi.org/10.1007/978-3-319-41501-7_67
Gonzalez, R.C., Woods, R.E.: Digital Image Processing. 4th Edn. MedData Interactive, Pearson (2018)
Guo, M., Yongzhao, D.: Classification of thyroid ultrasound standard plane images using ResNet-18 networks. In: IEEE 13th International Conference on Anti-counterfeiting, Security, and Identification (ASID). IEEE (2019)
He, K., Zhang, X., Ren, S., Sun, J.: Identity mappings in deep residual networks. In: Leibe, B., Matas, J., Sebe, N., Welling, M. (eds.) ECCV 2016. LNCS, vol. 9908, pp. 630–645. Springer, Cham (2016). https://doi.org/10.1007/978-3-319-46493-0_38
Huang, G., Liu, Z., Van Der Maaten, L., Weinberger, K.Q.: Densely connected convolutional networks. In: IEEE Conference on Computer Vision and Pattern Recognition (CVPR), pp. 2261–2269 (2017)
Huang, Z., Zhu, X., Ding, M., Zhang, X.: Medical image classification using a light-weighted hybrid neural network based on PCANet and DenseNet. IEEE Access 8, 24697–24712 (2020)
Kassani, S.H., Kassani, P.H., Wesolowski, M.J., Schneider, K.A., Deters, R.: Classification of histopathological biopsy images using ensemble of deep learning networks. arXiv preprint arXiv:1909.11870 (2019)
Kumar, A., Kim, J., Lyndon, D., Fulham, M., Feng, D.: An ensemble of fine-tuned convolutional neural networks for medical image classification. IEEE J. Biomed. Health Inform. 21(1), 31–40 (2016)
Lakshmi, S., Vijayasenan, D., Sumam, D.S., Sreeram, S., Suresh, P.K.: An integrated deep learning approach towards automatic evaluation of Ki-67 labeling index. In: Proceedings of the TENCON, IEEE Region 10 Conference (TENCON), Kochi, India, 17–20 October, pp. 2310–2314 (2019)
Lim, K.-T., Park, S.-H., Kim, J., Seonwoo, H., Choung, P.-H., Chung, J.H.: Cell image processing method for automatic cell pattern recognition and morphological analysis of mesenchymal stem cells-an algorithm for cell classification and adaptive brightness correction. J. Biosyst. Eng. 38, 55–63 (2013)
Lotufo, R.A., Rittner, L., Audigier, R., Machado, R.C., Saúde, A.V.: In: Deserno, T. (eds.) Biomedical Image Processing. Biological and Medical Physics, Biomedical Engineering. Springer, Heidelberg (2010). https://doi.org/10.1007/978-3-642-15816-2
Marques, G., Deevyankar, A., de la Torre DÃez, I.: Automated medical diagnosis of COVID-19 through EfficientNet convolutional neural network. Appl. Soft Comput. 96, 106691 (2020)
Negahbani, F., Sabzi, R., Pakniyat Jahromi, B., et al.: PathoNet introduced as a deep neural network backend for evaluation of Ki-67 and tumor-infiltrating lymphocytes in breast cancer. Sci. Rep. 11, 8489 (2021)
Ragab, H.M., Samy, N., Afify, M., Maksoud, Nl. Abd El., Shaaban, H.A.M.: Assessment of Ki-67 as a potential biomarker in patients with breast cancer. J. Genet. Eng. Biotechnol. 16, 479–484 (2018)
Rubin, J., Parvaneh, S., Rahman, A., Conroy, B., Babaeizadeh, S.: Densely connected convolutional networks for detection of atrial fibrillation from short single-lead ECG recordings. J Electrocardiol. 51(6S), S18–S21 (2018)
Sandler, M., Howard, A., Zhu, M., Zhmoginov, A., Chen, L.-Ch.: MobileNetV2: inverted residuals and linear bottlenecks. In: Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition (CVPR), pp. 4510–4520 (2018)
Saha, M., Chakraborty, C., Arun, I., et al.: An advanced deep learning approach for Ki-67 stained hotspot detection and proliferation rate scoring for prognostic evaluation of breast cancer. Sci. Rep. 7, 3213 (2017)
Shvets, A., Rakhlin, A., Kalinin, A.A., Iglovikov, V.: Automatic instrument segmentation in robot-assisted surgery using deep learning. In: 17th IEEE International Conference on Machine Learning and Applications (ICMLA). IEEE (2018)
Siegel, R.L., Miller, K.D., Fuchs, H.E., Jemal, A.: Cancer statistics, 2021. CA Cancer J. Clin. 71, 7–33 (2021)
Simonyan, K., Zisserman A.: Very deep convolutional networks for large-scale image recognition. In: 3rd International Conference on Learning Representations (ICLR) (2015)
Swiderska-Chadaj, Z., Gallego, J., Gonzalez-Lopez, L., Bueno, G.: Detection of Ki67 hot-spots of invasive breast cancer based on convolutional neural networks applied to mutual information of H&E and Ki67 whole slide images. Appl. Sci. 10, 7761 (2020)
Szegedy, C., Liu, W., Jia, Y., et al.: Going deeper with convolutions. In: Proceedings of the IEEE Conference on Computer Vision and Pattern Recognition (2015)
Tan, M., Quoc, L.: Efficientnet: Rethinking model scaling for convolutional neural networks. In: International Conference on Machine Learning. PMLR (2019)
Valkonen, M., Isola, J., Ylinen, O., Muhonen, V., Saxlin, A., Tolonen, T., Nykter, M., Ruusuvuori, P.: Cytokeratin-supervised deep learning for automatic recognition of epithelial cells in breast cancers stained for ER, PR, and Ki-67. IEEE Trans. Med. Imaging. 39(2), 534–542 (2020)
Xie, S., Zheng, X., Chen, Y., et al.: Artifact removal using improved GoogLeNet for sparse-view CT reconstruction. Sci Rep. 8(1), 6700 (2018)
Yang, Q., Yan, P., Kalra, M.K., Wang, G.: CT image denoising with perceptive deep neural networks. ArXiv170207019 Cs [Internet] (2017)
Acknowledgement
This work was supported by the statutory funds of the Department of Computational Intelligence, Faculty of Computer Science and Management, Wroclaw University of Science and Technology.
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Zawisza, A., Tabakov, M., Karanowski, K., Galus, K. (2021). Morphological Analysis of Histopathological Images Using Deep Learning. In: Wojtkiewicz, K., Treur, J., Pimenidis, E., Maleszka, M. (eds) Advances in Computational Collective Intelligence. ICCCI 2021. Communications in Computer and Information Science, vol 1463. Springer, Cham. https://doi.org/10.1007/978-3-030-88113-9_11
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DOI: https://doi.org/10.1007/978-3-030-88113-9_11
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