Abstract
Motor proteins, such as myosin and kinesin, are biological molecular motors involved in force generation and material transport in living cells. Motor proteins and their associated cytoskeletal filaments, such as actin filaments and microtubules, have been utilized for active transport in engineered systems. In controlling the active transport, external forces via electric fields or fluid flow were commonly used. A drawback of using external force is that the external force can cause detachment of microtubules from gliding surfaces. Detachment leads to loss of cargo or sparse surface density of microtubules, thus limiting the availability of external forces. Detachment should be minimized. In doing so, detailed observation on the process of detachment would be helpful. However, due to its limited spatial and temporal resolution, experimental investigations are hampered. Here, we show a simulation study for the detachment of microtubules gliding over surfaces coated with kinesin motors by an external force. Owing to the computer simulation’s high spatial and time resolution, two modes of detachment were found. Detailed processes of the two modes were revealed, which would be useful to diminish detachment.
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References
Saper, G., Hess, H.: Synthetic systems powered by biological molecular motors. Chem. Rev. 120(1), 288–309 (2020). https://doi.org/10.1021/acs.chemrev.9b00249
Lin, C.T., Kao, M.T., Kurabayashi, K., Meyhofer, E.: Self-contained, biomolecular motor-driven protein sorting and concentrating in an ultrasensitive microfluidic chip. Nano. Lett. 8(4), 1041–1046 (2008). https://doi.org/10.1021/nl072742x
Fischer, T., Agarwal, A., Hess, H.: A smart dust biosensor powered by kinesin motors. Nat. Nanotechnol. 4(3), 162–166 (2009). https://doi.org/10.1038/nnano.2008.393
Lard, M., et al.: Ultrafast molecular motor driven nanoseparation and biosensing. Biosens. Bioelectron. 48, 145–152 (2013). https://doi.org/10.1016/j.bios.2013.03.071
Nakano, T., Moore, M.J., Wei, F., Vasilakos, A.V., Shuai, J.: Molecular communication and networking: opportunities and challenges. IEEE Trans. Nanobioscience 11(2), 135–148 (2012). https://doi.org/10.1109/TNB.2012.2191570
Farsad, N., Yilmaz, H.B., Eckford, A., Chae, C.B., Guo, W.: A comprehensive survey of recent advancements in molecular communication. IEEE Commun. Surv. Tutorials 18(3), 1887–1919 (2016). https://doi.org/10.1109/COMST.2016.2527741
Van Den Heuvel, M.G.L., De Graaff, M.P., Dekker, C.: Molecular sorting by electrical steering of microtubules in kinesin-coated channels. Science 312(5775), 910–914 (2006). https://doi.org/10.1126/science.1124258
Agayan, R.R., et al.: Optimization of isopolar microtubule arrays. Langmuir 29(7), 2265–2272 (2013). https://doi.org/10.1021/la303792v
Isozaki, N., Shintaku, H.,Kotera, H., Hawkins, T.L., Ross, J. L., Yokokawa, R.: M I C R O R O B O T S Control of molecular shuttles by designing electrical and mechanical properties of microtubules. [Online]. Available http://robotics.sciencemag.org/ (2017)
Bassir Kazeruni, N.M., Rodriguez, J.B., Saper, G., Hess, H.: Microtubule detachment in gliding motility assays limits the performance of kinesin-driven molecular shuttless. Langmuir. 36(27), 7901–7907 (2020). https://doi.org/10.1021/acs.langmuir.0c01002
Nitta, T., Tanahashi, A., Hirano, M., Hess, H.: Simulating molecular shuttle movements: Towards computer-aided design of nanoscale transport systems. Lab. Chip. 6(7), 881 (2006). https://doi.org/10.1039/b601754a
Nitta, T., Tanahashi, A., Hirano, M.: In silico design and testing of guiding tracks for molecular shuttles powered by kinesin motors. Lab. Chip. 10(11), 1447 (2010). https://doi.org/10.1039/b926210e
Ishigure, Y., Nitta, T.: Understanding the Guiding of Kinesin/Microtubule-Based Microtransporters in Microfabricated Tracks. Langmuir 30(40), 12089–12096 (2014). https://doi.org/10.1021/la5021884
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Sweet, M., Nitta, T. (2021). Detachment of Microtubules Driven by Kinesin Motors from Track Surfaces Under External Force. In: Nakano, T. (eds) Bio-Inspired Information and Communications Technologies. BICT 2021. Lecture Notes of the Institute for Computer Sciences, Social Informatics and Telecommunications Engineering, vol 403. Springer, Cham. https://doi.org/10.1007/978-3-030-92163-7_16
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DOI: https://doi.org/10.1007/978-3-030-92163-7_16
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