Abstract
Sample preparation is an important step in any biochemical protocol, and several algorithms are known for automating them on a digital microfluidic (DMF) lab-on-a-chip (LoC). On-chip dilution of a sample on a DMF biochip involves several mix-split steps, which often suffer from the inaccuracies caused by unbalanced splitting of micro-fluid droplets. Also, error minimization is essential because of the limited availability of the stock solutions and costly reagents. In this work, we analyze the performance of two dilution algorithms Min-Mix (Thies et al., 2008) and DMRW (Roy et al., 2010) in the presence of volumetric errors that may occur during the splitting process. Our analysis exposes many interesting error behaviors and indicates possible solutions to correct them in a cyber-physical microfluidic platform.
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References
Chakrabarty, K., Xu, T.: Digital Microfluidic Biochips: Design and Optimization. CRC Press (2010)
Ren, H., Srinivasan, V., Fair, R.B.: Design and testing of an interpolating mixing architecture for electro wetting-based droplet-on-chip chemical dilution. In: Proc. Int. Conf. Solid-state Sensors, Actuators Microsyst. (Tranducers), pp. 619–622 (2003)
Fair, R.B., Srinivasan, V., Ren, H., Paik, P., Pamula, V.K., Pollack, M.G.: Electrowetting-based on-chip Sample processing for integrated microfluidics. In: Proc. IEEE IEDM Tech. Dig., pp. 32.5.1–32.5.4 (2003)
Pollack, M.G., Shendorov, A.D., Fair, R.B.: Electrowetting-based actuation of droplets for integrated microfluidics. Lab on a Chip 2(1), 96–101 (2002)
Paik, P., Pamula, V.K., Pollack, M.G., Fair, R.B.: Electrowetting-based droplet mixers for microfluidic systems. Lab on a Chip 3(1), 28–33 (2003)
Srinivasan, V., Pamula, V., Pollack, M.G., Fair, R.B.: A digital microfluidic biosensor for multianalyte detection. In: Technical Digest MEMS 2003, pp. 327–330 (2003)
Theis, W., Urbanski, J.P., Thorsen, T., Amarasinghe, S.: Abstraction layers for scalable microfluidic biocomputing. Nat. Comput. 7(2), 255–275 (2008)
Roy, S., Bhattacharya, B.B., Chakrabarty, K.: Optimization of Dilution and Mixing of Biochips. IEEE TCAD 29(11), 1696–1708 (2010)
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Bera, N., Majumder, S., Bhattacharya, B.B. (2014). Analysis of Concentration Errors in Sample Dilution Algorithms on a Digital Microfluidic Biochip. In: Gupta, P., Zaroliagis, C. (eds) Applied Algorithms. ICAA 2014. Lecture Notes in Computer Science, vol 8321. Springer, Cham. https://doi.org/10.1007/978-3-319-04126-1_8
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DOI: https://doi.org/10.1007/978-3-319-04126-1_8
Publisher Name: Springer, Cham
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