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Change of Network Dynamics in a Neuro-robotic System

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Biomimetic and Biohybrid Systems (Living Machines 2014)

Part of the book series: Lecture Notes in Computer Science ((LNAI,volume 8608))

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Abstract

In the past, tetanic stimulation has been used in several different instances to induce changes in the firing patterns of neural networks in vitro. In this paper, we ran a new experimental campaign to verify if this protocol induced lasting changes and if those changes were predictable. We found out that our stimulation protocol led to different results in cortical and hippocampal preparations: in the first case, stronger connections were weakened, resulting in a reduction of bursting activity and late evoked response; in the case of hippocampal preparations, single strong connections underwent strong changes but, on average, remained unchanged. In both preparations, the geometry of induced changes remains largely uncorrelated with the actual site of stimulation delivery.

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References

  1. Beggs, J.M., Plenz, D.: Neuronal avalanches in neocortical circuits. The Journal of Neuroscience 23, 11167–11177 (2003)

    Google Scholar 

  2. Wagenaar, D.A., Madhavan, R., Pine, J., Potter, S.M.: Controlling bursting in cortical cultures with closed-loop multi-electrode stimulation. The Journal of Neuroscience 25, 680–688 (2005)

    Article  Google Scholar 

  3. Eytan, D., Marom, S.: Dynamics and effective topology underlying synchronization in networks of cortical neurons. The Journal of Neuroscience 26, 8465–8476 (2006)

    Article  Google Scholar 

  4. Wagenaar, D.A., Pine, J., Potter, S.M.: An extremely rich repertoire of bursting patterns during the development of cortical cultures. BMC Neuroscience 7, 11 (2006)

    Article  Google Scholar 

  5. Pasquale, V., Massobrio, P., Bologna, L., Chiappalone, M., Martinoia, S.: Self-organization and neuronal avalanches in networks of dissociated cortical neurons. Neuroscience 153, 1354–1369 (2008)

    Article  Google Scholar 

  6. Raichman, N., Ben-Jacob, E.: Identifying repeating motifs in the activation of synchronized bursts in cultured neuronal networks. Journal of Neuroscience Methods 170, 96–110 (2008)

    Article  Google Scholar 

  7. Van Pelt, J., Corner, M.A., Wolters, P.S., Rutten, W.L.C., Ramakers, G.J.A.: Longterm stability and developmental changes in spontaneous network burst firing patterns in dissociated rat cerebral cortex cell cultures on multi-electrode arrays. Neurosci. Letters 361, 86–89 (2004)

    Article  Google Scholar 

  8. Bologna, L.L., Nieus, T., Tedesco, M., Chiappalone, M., Benfenati, F., Martinoia, S.: Low-frequency stimulation enhances burst activity in cortical cultures during development. Neuroscience (2010)

    Google Scholar 

  9. Marom, S., Shahaf, G.: Development, learning and memory in large random networks of cortical neurons: lessons beyond anatomy. Quarterly Reviews of Biophysics 35, 63–87 (2002)

    Article  Google Scholar 

  10. Bonifazi, P., Ruaro, M.E., Torre, V.: Statistical properties of information processing in neuronal networks. Eur. J. Neurosci. 22, 2953–2964 (2005)

    Article  Google Scholar 

  11. Chiappalone, M., Vato, A., Berdondini, L., Koudelka, M., Martinoia, S.: Network dynamics and synchronous activity in cultured cortical neurons. Int. J. Neural. Syst. 17, 87–103 (2007)

    Article  Google Scholar 

  12. Maeda, E., Robinson, H.P.C., Kawana, A.: The mechanism of generation and propagation of synchronized bursting in developing networks of cortical neurons. The Journal of Neuroscience: the Official Journal of the Society for Neuroscience 15, 6834–6845 (1995)

    Google Scholar 

  13. Dal Maschio, M., Difato, F., Beltramo, R., Blau, A., Benfenati, F., Fellin, T.: Simultaneous two-photon imaging and photo-stimulation with structured light illumination. Optic Express 18, 18720–18731 (2010)

    Article  Google Scholar 

  14. Frega, M., Tedesco, M., Massobrio, P., Pesce, M., Martinoia, S.: 3D neuronal networks coupled to micro-electrode arrays: an innovative in vitro experimental model to study network dynamics. In: 8th International Meeting on Substrate-Integrated Microelectrode Arrays, pp. NMI

    Google Scholar 

  15. Gramowski, A., Jügelt, K., Stüwe, S., Schulze, R., McGregor, G.P., Wartenberg-Demand, A., Loock, J., Schröder, O., Weiss, D.G.: Functional screening of traditional antidepressants with primary cortical neuronal networks grown on multielectrode neurochips. European Journal of Neuroscience 24, 455–465 (2006)

    Article  Google Scholar 

  16. Keefer, E.W., Gramowski, A., Gross, G.W.: NMDA receptor-dependent periodic oscillations in cultured spinal cord networks. Journal of Neurophysiology 86, 3030–3042 (2001)

    Google Scholar 

  17. Jimbo, Y., Robinson, H.P.C., Kawana, A.: Strengthening of synchronized activity by tetanic stimulation in cortical cultures: application of planar electrode arrays. IEEE Transactions on Biomedical Engineering 45, 1297–1304 (1998)

    Article  Google Scholar 

  18. Jimbo, Y., Tateno, Y., Robinson, H.P.C.: Simultaneous induction of pathway-specific potentiation and depression in networks of cortical neurons. Biophysical Journal 76, 670–678 (1999)

    Article  Google Scholar 

  19. Stegenga, J., le Feber, J., Marani, E., Rutten, W.L.: Phase-dependent effects of stimuli locked to oscillatory activity in cultured cortical networks. Biophys. J. 98, 2452–2458 (2010)

    Article  Google Scholar 

  20. Tateno, T., Jimbo, Y.: Activity-dependent enhancement in the reliability of correlated spike timings in cultured cortical neurons. Biological Cybernetics 80, 45–55 (1999)

    Article  MATH  Google Scholar 

  21. Madhavan, R., Chao, Z.C., Potter, S.M.: Plasticity of recurring spatiotemporal activity patterns in cortical networks. Physical Biology 4, 181–193 (2007)

    Article  Google Scholar 

  22. Shahaf, G., Marom, S.: Learning in networks of cortical neurons. The Journal of Neuroscience: the Official Journal of the Society for Neuroscience 21, 8782–8788 (2001)

    Google Scholar 

  23. le Feber, J., Stegenga, J., Rutten, W.L.: The effect of slow electrical stimuli to achieve learning in cultured networks of rat cortical neurons. PloS One 5, e8871 (2010)

    Google Scholar 

  24. Tessadori, J., Mulas, M., Martinoia, S., Chiappalone, M.: A neuro-robotic system to investigate the computational properties of neuronal assemblies. In: 2012 4th IEEE RAS & EMBS International Conference on Biomedical Robotics and Biomechatronics (BioRob), pp. 332–337. IEEE (2012)

    Google Scholar 

  25. Tessadori, J., Venuta, D., Kumar, S.S., Bisio, M., Pasquale, V., Chiappalone, M.: Embodied neuronal assemblies: A closed-loop environment for coding and decoding studies. In: 2013 6th International IEEE/EMBS Conference on Neural Engineering (NER), pp. 899–902. IEEE (2013)

    Google Scholar 

  26. Tessadori, J., Venuta, D., Pasquale, V., Kumar, S.S., Chiappalone, M.: Encoding of stimuli in embodied neuronal networks. In: Lepora, N.F., Mura, A., Krapp, H.G., Verschure, P.F.M.J., Prescott, T.J. (eds.) Living Machines 2013. LNCS, vol. 8064, pp. 274–286. Springer, Heidelberg (2013)

    Chapter  Google Scholar 

  27. Gal, A., Marom, S.: Entrainment of the intrinsic dynamics of single isolated neurons by natural-like input. The Journal of neuroscience 33, 7912–7918 (2013)

    Article  Google Scholar 

  28. Mainen, Z.F., Sejnowski, T.J.: Reliability of spike timing in neocortical neurons. Science 268, 1503–1506 (1995)

    Article  Google Scholar 

  29. Frega, M., Pasquale, V., Tedesco, M., Marcoli, M., Contestabile, A., Nanni, M., Bonzano, L., Maura, G., Chiappalone, M.: Cortical cultures coupled to micro-electrode arrays: a novel approach to perform in vitro excitotoxicity testing. Neurotoxicology and Teratology 34, 116–127 (2012)

    Article  Google Scholar 

  30. Chiappalone, M., Vato, A., Berdondini, L., Koudelka-Hep, M., Martinoia, S.: Network dynamics and synchronous activity in cultured cortical neurons. International Journal of Neural Systems 17, 87–103 (2007)

    Article  Google Scholar 

  31. Madhavan, R., Chao, Z.C., Potter, S.M.: Plasticity of recurring spatiotemporal activity patterns in cortical networks. Physical Biology 4, 181 (2007)

    Article  Google Scholar 

  32. Chiappalone, M., Massobrio, P., Martinoia, S.: Network plasticity in cortical assemblies. European Journal of Neuroscience 28, 221–237 (2008)

    Article  Google Scholar 

  33. Tessadori, J., Bisio, M., Martinoia, S., Chiappalone, M.: Modular neuronal assemblies embodied in a closed-loop environment: toward future integration of brains and machines. Frontiers in Neural Circuits 6 (2012)

    Google Scholar 

  34. Weihberger, O., Okujeni, S., Mikkonen, J.E., Egert, U.: Quantitative examination of stimulus-response relations in cortical networks in vitro. Journal of Neurophysiology 109, 1764–1774 (2013)

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia, Genova, Italy

    Irene Nava, Jacopo Tessadori & Michela Chiappalone

Authors
  1. Irene Nava
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  2. Jacopo Tessadori
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  3. Michela Chiappalone
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Editor information

Editors and Affiliations

  1. Universitat Pompeu Fabra, Barcelona, Spain

    Armin Duff

  2. University of Bristol, UK

    Nathan F. Lepora

  3. University of Pompeau Fabra, Barcelona, Spain

    Anna Mura

  4. University of Sheffield, Sheffield, UK

    Tony J. Prescott

  5. Universitat Pompeu Fabra and Catalan Institution for Research and Advanced Studies, Barcelona, Spain

    Paul F. M. J. Verschure

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© 2014 Springer International Publishing Switzerland

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Nava, I., Tessadori, J., Chiappalone, M. (2014). Change of Network Dynamics in a Neuro-robotic System. In: Duff, A., Lepora, N.F., Mura, A., Prescott, T.J., Verschure, P.F.M.J. (eds) Biomimetic and Biohybrid Systems. Living Machines 2014. Lecture Notes in Computer Science(), vol 8608. Springer, Cham. https://doi.org/10.1007/978-3-319-09435-9_20

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  • DOI: https://doi.org/10.1007/978-3-319-09435-9_20

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-09434-2

  • Online ISBN: 978-3-319-09435-9

  • eBook Packages: Computer ScienceComputer Science (R0)

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