Skip to main content

Towards Automated Cell Segmentation in Corneal Endothelium Images

  • Conference paper
Image Processing & Communications Challenges 6

Part of the book series: Advances in Intelligent Systems and Computing ((AISC,volume 313))

Abstract

This article addresses the problem of corneal endothelium image segmentation. The aim is an objective determination of boundaries between cells. This problem has not been solved yet as a fully automatic process, the majority of commercial software requires additional correction by an ophthalmologist.

In the paper there are described two approaches allowing to achieve the segmentation of cells that perform more accurate than standard implementations of Watershed algorithms. There is also proposed an algorithm to improve the existing cells division, based on matching proposed segmentation grid lines to the input image content.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ayala, G., Diaz, M., Martinez-Costa, L.: Granulometric moments and corneal endothelium status. Pattern RecognitionĀ 34(6), 1219ā€“1227 (2001)

    ArticleĀ  MATHĀ  Google ScholarĀ 

  2. Doughty, M.: The ambiguous coefficient of variation: Polymegethism of the corneal endothelium and central corneal thickness. International Contact Lens ClinicĀ 17(9-10) (1990)

    Google ScholarĀ 

  3. Doughty, M.: Concerning the symmetry of the ā€˜hexagonalā€™ cells of the corneal endothelium. Experimental Eye ResearchĀ 55(1), 145ā€“154 (1992)

    ArticleĀ  Google ScholarĀ 

  4. Gronkowska-Serafin, J., PiĆ³rkowski, A.: Corneal endothelial grid structure factor based on coefficient of variation of the cell sides lengths. In: Choras, R.S. (ed.) Image Processing and Communications Challenges 5. AISC, vol.Ā 233, pp. 13ā€“20. Springer, Heidelberg (2014)

    ChapterĀ  Google ScholarĀ 

  5. Habrat, K.: Binarization of corneal endothelial digital images. Masterā€™s thesis, AGH University of Science and Technology (2012)

    Google ScholarĀ 

  6. Khan, M.A.U., Niazi, M.K.K., Khan, M.A., Ibrahim, M.T.: Endothelial cell image enhancement using non-subsampled image pyramid. Information Technology JournalĀ 6(7), 1057ā€“1062 (2007)

    ArticleĀ  Google ScholarĀ 

  7. Mahzoun, M., Okazaki, K., Mitsumoto, H., Kawai, H., Sato, Y., Tamura, S., Kani, K.: Detection and complement of hexagonal borders in corneal endothelial cell image. Medical Imaging TechnologyĀ 14(1), 56ā€“69 (1996)

    Google ScholarĀ 

  8. Oblak, E., Doughty, M., Oblak, L.: A semi-automated assessment of cell size and shape in monolayers, with optional adjustment for the cell-cell border width-application to human corneal endothelium. Tissue and CellĀ 34(4), 283ā€“295 (2002)

    ArticleĀ  Google ScholarĀ 

  9. Ogiela, M., Tadeusiewicz, R.: Artificial intelligence methods in shape feature analysis of selected organs in medical images. Image Processing and CommunicationsĀ 6, 3ā€“11 (2000)

    Google ScholarĀ 

  10. Piorkowski, A., Gronkowska-Serafin, J.: Analysis of corneal endothelial image using classic image processing methods. In: KOWBAN - XVIII The Computer-Aided Scientific Research, The Works of Wroclaw Scientific Society, B, Wroclawskie Towarzystwo Naukowe, vol.Ā 217, pp. 283ā€“290 (2011)

    Google ScholarĀ 

  11. Piorkowski, A., Gronkowska-Serafin, J.: Selected issues of corneal endothelial image segmentation. Journal of Medical Informatics and TechnologiesĀ 17, 239ā€“245 (2011)

    Google ScholarĀ 

  12. Placzek, B.: Rough sets in identification of cellular automata for medical image processing. Journal of Medical Informatics and TechnologiesĀ 22, 161ā€“168 (2013)

    Google ScholarĀ 

  13. Rao, G.N., Lohman, N., Aquavella, L., Cell, J.: size-shape relationships in corneal endothelium. Investigative Ophthalmology and Visual ScienceĀ 22(2), 271ā€“274 (1982)

    Google ScholarĀ 

  14. Sanchez-Marin, F.: Automatic segmentation of contours of corneal cells. Computers in Biology and MedicineĀ 29(4), 243ā€“258 (1999)

    ArticleĀ  Google ScholarĀ 

  15. Szostek, K., Gronkowska-Serafin, J., Piorkowski, A.: Problems of corneal endothelial image binarization. Schedae InformaticaeĀ 20, 211ā€“218 (2011)

    Google ScholarĀ 

  16. Zapter, V., Martinez-Costa, L., Ayala, G.: A granulometric analysis of specular microscopy images of human corneal endothelia. Computer Vision and Image UnderstandingĀ 97(3), 297ā€“314 (2005)

    ArticleĀ  Google ScholarĀ 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Adam PiĆ³rkowski .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

Ā© 2015 Springer International Publishing Switzerland

About this paper

Cite this paper

PiĆ³rkowski, A., Gronkowska-Serafin, J. (2015). Towards Automated Cell Segmentation in Corneal Endothelium Images. In: Choraś, R. (eds) Image Processing & Communications Challenges 6. Advances in Intelligent Systems and Computing, vol 313. Springer, Cham. https://doi.org/10.1007/978-3-319-10662-5_22

Download citation

  • DOI: https://doi.org/10.1007/978-3-319-10662-5_22

  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-10661-8

  • Online ISBN: 978-3-319-10662-5

  • eBook Packages: EngineeringEngineering (R0)

Publish with us

Policies and ethics