Abstract
Based on the need for small scale compliant devices for use in medical robotics, there is increasing interest in the development of muscle actuated biobots. Such biobots are traditionally fabricated using nondegradable, synthetic polymers; however, such substrates require micro-patterning and additional treatments in order to promote cellular adhesion and induce cellular alignment. By using an organic substrate, such steps can be eliminated, and degradable scaffolds can be produced. Here we present a manufacturing process and culture conditions for fabrication of living machines using electrochemically compacted and aligned collagen (ELAC) as a scaffold. Using collagen as a scaffold results in a completely organic device. Milli-scale scaffolds were seeded with primary cardiomyocytes isolated from chick embryos and electrically stimulated to induce movement.
V.A. Webster—This material is based upon work supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE-0951783. This study was also funded in part by grants from the National Science Foundation (Grant Number DMR-1306665) and National Institute of Health (Grant Number R01 AR063701). Any opinion, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation.
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Webster, V.A., Hawley, E.L., Akkus, O., Chiel, H.J., Quinn, R.D. (2015). Fabrication of Electrocompacted Aligned Collagen Morphs for Cardiomyocyte Powered Living Machines. In: Wilson, S., Verschure, P., Mura, A., Prescott, T. (eds) Biomimetic and Biohybrid Systems. Living Machines 2015. Lecture Notes in Computer Science(), vol 9222. Springer, Cham. https://doi.org/10.1007/978-3-319-22979-9_43
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