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Type P63 Digitized Color Images Performs Better Identification than Other Stains for Ovarian Tissue Analysis

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Articulated Motion and Deformable Objects (AMDO 2016)

Part of the book series: Lecture Notes in Computer Science ((LNIP,volume 9756))

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Abstract

Ovarian reproductive tissues are responsible for human reproduction. It is important for the pathology experts to perform routine examination for ovarian reproductive tissues to prescribe necessary treatments for women who face conceiving complications. Manual microscopic analysis is considered the best analysis approach for experts in the laboratory as existing scanning modalities do not provide satisfactory results for identification. Due to longer processing time and observation variability between experts computer based approaches have become popular as it can reduce time and can identify the reproductive tissue accurately. In this paper a new modified approach is presented and comparative analysis were performed using existing computer based approaches on three different types of digitized images acquired from microscopic biopsy slides. Proposed new modified approach indicates acceptable accuracy rate in comparison to manual identification approach to analyze ovarian reproductive tissues.

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References

  1. Skodras, A., Giannarou, S., Fenwick, M., Franks, S., Stark, J., Hardy, K.: Object recognition in the ovary: quantification of oocytes from microscopic images. In: 2009 16th International Conference on Digital Signal Processing, pp. 1–6 (2009)

    Google Scholar 

  2. Kiruthika, V., Ramya, M.: Automatic segmentation of ovarian follicle using K-means clustering. In: 2014 Fifth International Conference on Signal and Image Processing (ICSIP), pp. 137–141 (2014)

    Google Scholar 

  3. Muskhelishvili, L., Wingard, S.K., Latendresse, J.R.: Proliferating cell nuclear antigen—a marker for ovarian follicle counts. Toxicol. Pathol. 33, 365–368 (2005)

    Article  Google Scholar 

  4. Lamprecht, M.R., Sabatini, D.M., Carpenter, A.E.: CellProfilerâ„¢: free, versatile software for automated biological image analysis. Biotechniques 42, 71 (2007)

    Article  Google Scholar 

  5. Magee, D., Treanor, D., Crellin, D., Shires, M., Smith, K., Mohee, K., et al.: Colour normalisation in digital histopathology images (2009)

    Google Scholar 

  6. Picut, C.A., Swanson, C.L., Scully, K.L., Roseman, V.C., Parker, R.F., Remick, A.K.: Ovarian follicle counts using proliferating cell nuclear antigen (PCNA) and semi-automated image analysis in rats. Toxicol. Pathol. 36, 674–679 (2008)

    Article  Google Scholar 

  7. Mouroutis, T., Roberts, S.J., Bharath, A.A.: Robust cell nuclei segmentation using statistical modelling. Bioimaging 6, 79–91 (1998)

    Article  Google Scholar 

  8. Bucci, T.J., Bolon, B., Warbritton, A.R., Chen, J.J., Heindel, J.J.: Influence of sampling on the reproducibility of ovarian follicle counts in mouse toxicity studies. Reprod. Toxicol. 11, 689–696 (1997)

    Article  Google Scholar 

  9. Bolon, B., Bucci, T.J., Warbritton, A.R., Chen, J.J., Mattison, D.R., Heindel, J.J.: Differential follicle counts as a screen for chemically induced ovarian toxicity in mice: results from continuous breeding bioassays. Toxicol. Sci. 39, 1–10 (1997)

    Article  Google Scholar 

  10. Kelsey, T.W., Caserta, B., Castillo, L., Wallace, W.H.B., Gonzálvez, F.C.: Proliferating cell nuclear antigen (PCNA) allows the automatic identification of follicles in microscopic images of human ovarian tissue. arXiv preprint arXiv:1008.3798 (2010)

  11. Sazzad, T., Armstrong, L., Tripathy, A.: An automated detection process to detect ovarian tissues using type P63 digitized color images. In: 2015 IEEE 27th International Conference on Tools with Artificial Intelligence (ICTAI), pp. 278–285 (2015)

    Google Scholar 

  12. Sazzad, T., Armstrong, L., Tripathy, A.: An automated approach to detect human ovarian tissues using type P63 counter stained histopathology digitized color images. In: IEEE-EMBS International Conference on Biomedical and Health Informatics (BHI), pp. 25– 28 (2016)

    Google Scholar 

  13. Sazzad, T., Armstrong, L., Tripathy, A.: A comparative study of computerized approaches for type P63 ovarian tissues using histopathology digitized color images. In: 10th International Conference on Computer Graphics, Visualization, Computer Vision and Image Processing (CGVCVIP 2016), Portugal (2016)

    Google Scholar 

  14. Sertel, O., Catalyurek, U.V., Shimada, H., Guican, M.: Computer-aided prognosis of neuroblastoma: detection of mitosis and karyorrhexis cells in digitized histological images. In: Engineering in Medicine and Biology Society 2009, EMBC 2009, pp. 1433–1436. Annual International Conference of the IEEE (2009)

    Google Scholar 

  15. Azevedo, L., Faustino, A.M., Tavares, J.M.R.: Segmentation and 3D reconstruction of animal tissues in histological images. In: Tavares, J.M.R.S., Natal Jorge, R.M. (eds.) Computational and Experimental Biomedical Sciences: Methods and Applications, pp. 193–207. Springer, Switzerland (2015)

    Google Scholar 

  16. Sazzad, T., Armstrong, L., Tripathy, A.: Type P63 non-counter stained digitized color images performs better identification than other stains for ovarian tissue analysis. In: 2016 20th International Conference on Information Visualisation (2016)

    Google Scholar 

  17. Matthews, J., Altman, D.G., Campbell, M., Royston, P.: Analysis of serial measurements in medical research. BMJ: Br. Med. J. 300, 230 (1990)

    Article  Google Scholar 

  18. Nock, R., Nielsen, F.: Statistical region merging. IEEE Trans. Pattern Anal. Mach. Intell. 26, 1452–1458 (2004)

    Article  Google Scholar 

  19. Sazzad, T.S., Islam, S., Mamun, M.M.R.K., Hasan, M.Z.: Establishment of an efficient color model from existing models for better gamma encoding in image processing. Int. J. Image Process. (IJIP) 7(90), 90–100 (2013)

    Google Scholar 

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Acknowledgement

The authors would like to thank Assistant Professor and head of the department (Pathology) and domain expert Doctor Sadequel Islam Talukder (MBBS, M.Phil (Pathology), Shaheed Sayed Nazrul Islam Medical College, Kishoreganj, Bangladesh) for providing the test images, annotated images and necessary feature information.

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Correspondence to T. M. Shahriar Sazzad .

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Shahriar Sazzad, T.M., Armstrong, L.J., Tripathy, A.K. (2016). Type P63 Digitized Color Images Performs Better Identification than Other Stains for Ovarian Tissue Analysis. In: Perales, F., Kittler, J. (eds) Articulated Motion and Deformable Objects. AMDO 2016. Lecture Notes in Computer Science(), vol 9756. Springer, Cham. https://doi.org/10.1007/978-3-319-41778-3_5

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  • DOI: https://doi.org/10.1007/978-3-319-41778-3_5

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  • Publisher Name: Springer, Cham

  • Print ISBN: 978-3-319-41777-6

  • Online ISBN: 978-3-319-41778-3

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