Abstract
Among neurodegenerative diseases, Alzheimer Disease (AD) represents one of the most widespread pathologies, for which an early diagnosis is still missing. A peculiar expression of an altered conformational isoform of p53 protein was reported to be a potential biomarker able to distinguish AD subjects from healthy population, quantifiable using a blood-based enzyme-linked immunosorbent assay (ELISA). In order to overcome ELISA limitations, related to reliability and to improve sensitivity, this study aimed to realize a low cost highly sensitive portable point-of-care (PoC) testing system based on screen printed electrochemical sensors (SPES). The development of the platform specifically included both the design of the sensing probe and of the electronic circuit devoted to the conditioning and acquisition of the transduced electric signal. Preliminary testing of the circuit were performed recording changes in the conductivity of NaCl solution and quantifying electrodes coating with antibodies using Electrochemical Impedance Spectroscopy (EIS) principle. Results obtained with saline solution, showed the ability of the circuit to give the best response corresponding to low changes in NaCl concentration (sensitivity 0.2 mA/(mg/ml)), suggesting a good sensitivity of the platform. Findings obtained from EIS showed the ability of the circuit to discriminate between different concentrations of antibodies coatings (sensitivity 80 µA/µg). Preliminary calibration of the sensor using interleukin IL-8 showed an increase in the maximum peak of current proportional to the increase of protein concentration, achieving a sensitivity of 35 µA/ng and reporting also a good value of reproducibility, really promising in the perspective of lower values of proteins concentrations.
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Z. Svobodova, M. Reza Mohamadi, B. Jankovicova, H. Esselmann, R. Verpillot, M. Otto, M. Taverna, J. Wiltfang, J.-L. Viovy, Z. Bilkova, Development of a magnetic immunosorbent for on-chip preconcentration of amyloid beta isoforms: representatives of Alzheimer’s disease biomarkers. Biomicrofluidics 6(2), 24126–2412612 (2012)
C. Humpel, Identifying and validating biomarkers for Alzheimer’s disease. Trends Biotechnol. 29(1), 26–32 (2011)
D. Uberti, C. Lanni, T. Carsana, S. Francisconi, C. Missale, M. Racchi, S. Govoni, M. Memo, Identification of a mutant-like conformation of p53 in fibroblasts from sporadic Alzheimer’s disease patients. Neurobiol. Aging 27(9), 1193–1201 (2006)
C. Lanni, M. Racchi, G. Mazzini, A. Ranzenigo, R. Polotti, E. Sinforiani, L. Olivari, M. Barcikowska, M. Styczynska, J. Kuznicki, A. Szybinska, S. Govoni, M. Memo, D. Uberti, Conformationally altered p53: a novel Alzheimer’s disease marker? Mol. Psychiatry 13(6), 641–647 (2008)
L. Buizza, G. Cenini, C. Lanni, G. Ferrari-Toninelli, C. Prandelli, S. Govoni, E. Buoso, M. Racchi, M. Barcikowska, M. Styczynska, A. Szybinska, D.A. Butterfield, M. Memo, D. Uberti, Conformational altered p53 as an early marker of oxidative stress in Alzheimer’s disease. PLoS ONE 7(1), e29789 (2012)
A.P. Dhawan, W.J. Heetderks, M. Pavel, S. Acharya, M. Akay, A. Mairal, B. Wheeler, C.C. Dacso, T. Sunder, N. Lovell, M. Gerber, M. Shah, S.G. Senthilvel, M.D. Wang, B. Bhargava, Current and future challenges in point-of-care technologies: a paradigm-shift in affordable global healthcare with personalized and preventive medicine. Transl. Eng. Health Med., IEEE J. 3, 1–10 (2015)
D. Polese, A. Convertino, L. Maiolo, A. Ferrone, L. Pazzini, M. Marrani, F. Maita, A. Pecora, G. Fortunato, G. Fiaschi, Investigation on nanostructured biosensor for Biotin detection. Sensors, 2014 IEEE, pp. 1627–1630, 2014
S. Feng, L.E. Roseng, T. Dong, Quantitative detection of Escherichia coli and measurement of urinary tract infection diagnosis possibility by use of a portable, handheld sensor. Medical Measurements and Applications (MeMeA), 2015 IEEE International Symposium on, pp. 586–589, 2015
M.M.S. Silva, a.C.M.S. Dias, M.T. Cordeiro, E. Marques, M.O.F. Goulart, R.F. Dutra, A thiophene-modified screen printed electrode for detection of dengue virus NS1 protein. Talanta 128, 505–510 (2014)
R. Elshafey, A.C. Tavares, M. Siaj, M. Zourob, Electrochemical impedance immunosensor based on gold nanoparticles-protein G for the detection of cancer marker epidermal growth factor receptor in human plasma and brain tissue. Biosens. Bioelectron. 50, 143–149 (2013)
Y.H. Yun, B.K. Lee, J.S. Choi, S. Kim, B. Yoo, Y.S. Kim, K. Park, Y.W. Cho, A glucose sensor fabricated by piezoelectric inkjet printing of conducting polymers and bienzymes. Anal. Sci. 27(4), 375 (2011)
A.M. Pernia, M.J. Prieto, I.C. Orille, J.A. Martin-Ramos, A. Costa-Garcia, Development of Optimized Screen-Printed Immunosensors. Instrum. Measur., IEEE Trans. 58(7), 2181–2188 (2009)
R.A.S. Couto, J.L.F.C. Lima, M.B. Quinaz, Recent developments, characteristics and potential applications of screen-printed electrodes in pharmaceutical and biological analysis. Talanta 146, 801–814 (2016)
V. Escamilla-Gómez, D. Hernández-Santos, M.B. González-García, J.M. Pingarrón-Carrazón, A. Costa-García, Simultaneous detection of free and total prostate specific antigen on a screen-printed electrochemical dual sensor. Biosens. Bioelectron. 24, 2678–2683 (2009)
Y.-F. Liang, C.-Y. Huang, B.-D. Liu, A voltammetry potentiostat design for large dynamic range current measurement. Intelligent Computation and Bio-Medical Instrumentation (ICBMI), 2011 International Conference on, pp. 260–263, 2011
G. Martínez-Paredes, M.B. González-García, A. Costa-García, Genosensor for detection of four pneumoniae bacteria using gold nanostructured screen-printed carbon electrodes as transducers. Sens. Actuators, B Chem. 149, 329–335 (2010)
P. Kara, A. de la Escosura-Muñiz, M. Maltez-da Costa, M. Guix, M. Ozsoz, A. Merkoçi, Aptamers based electrochemical biosensor for protein detection using carbon nanotubes platforms. Biosens. Bioelectron. 26, 1715–1718 (2010)
B. Jeong, R. Akter, O.H. Han, C.K. Rhee, Increased electrocatalyzed performance through dendrimer–encapsulated gold nanoparticles and carbon nanotube–assisted multiple bienzymatic labels: highly sensitive electrochemical immunosensor for protein detection. Anal. Chem. 85(3), 1784–1791 (2013)
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Tonello, S., Serpelloni, M., Lopomo, N.F., Abate, G., Uberti, D.L., Sardini, E. (2018). Preliminary Study of a Low-Cost Point-of-Care Testing System Using Screen-Printed Biosensors for Early Biomarkers Detection Related to Alzheimer Disease. In: Andò, B., Baldini, F., Di Natale, C., Marrazza, G., Siciliano, P. (eds) Sensors. CNS 2016. Lecture Notes in Electrical Engineering, vol 431. Springer, Cham. https://doi.org/10.1007/978-3-319-55077-0_32
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